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A cell culture assay is any method used to assess the cytotoxicity of a material. [1] [2] This refers to the in vitro assessment of a material to determine whether it releases toxic chemicals in the cell. It also determines if the quantity is sufficient to kill cells, either directly or indirectly, through the inhibition of cell metabolic pathways.
In vitro toxicity testing is the scientific analysis of the toxic effects of chemical substances on cultured bacteria or mammalian cells. [1] In vitro (literally 'in glass') testing methods are employed primarily to identify potentially hazardous chemicals and/or to confirm the lack of certain toxic properties in the early stages of the development of potentially useful new substances such as ...
Two populations of cells are cultivated in cell culture.One of the cell populations is fed with growth medium containing normal amino acids.In contrast, the second population is fed with growth medium containing amino acids labeled with stable (non-radioactive) heavy isotopes.
Cytotoxicity can also be monitored using 3-(4, 5-Dimethyl-2-thiazolyl)-2, 5-diphenyl-2H-tetrazolium bromide or with 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT), which yields a water-soluble product, or the MTS assay. This assay measures the reducing potential of the cell using a colorimetric reaction.
The MP4 file format defined some extensions over the ISO Base Media File Format to support MPEG-4 visual/audio codecs and various MPEG-4 Systems features such as object descriptors and scene descriptions. Some of these extensions are also used by other formats based on the ISO base media file format (e.g., 3GP). [1]
[2] [3] This is the major advantage in using the culture tissue assay to detect toxicity in PMC patients. [2] Even though clinical laboratories have tried to use an assay microtiter plate enzyme-linked immunosorbent assay (ELISA) and other techniques to detect the cytotoxic activity of toxin B in the feces of PMC patients, the results are not ...
Complement-dependent cytotoxicity (CDC) is an effector function of IgG and IgM antibodies.When they are bound to surface antigen on target cell (e.g. bacterial or viral infected cell), the classical complement pathway is triggered by bonding protein C1q to these antibodies, resulting in formation of a membrane attack complex (MAC) and target cell lysis.
A high throughput assay can be either an endpoint or a kinetic assay usually done on an automated platform in 96-, 384- or 1536-well microplate formats (High Throughput Screening). Such assays are able to test large number of compounds or analytes or make functional biological readouts in response to a stimuli and/or compounds being tested. [6]
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