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[2]: 518 The microbiologist examines the appearance of the colony, noting specific features such as size, colour, shape, consistency, and opacity. [1]: 165–8 A hand lens or magnifying glass may be used to view colonies in greater detail. [3]: 96 The opacity of a microbial colony can be described as transparent, translucent, or opaque.
A coccus (plural cocci, from the Latin coccinus (scarlet) and derived from the Greek kokkos (berry)) is any microorganism (usually bacteria) [1] whose overall shape is spherical or nearly spherical. [2] [3] [4] Coccus refers to the shape of the bacteria, and can contain multiple genera, such as staphylococci or streptococci. Cocci can grow in ...
An agar plate – an example of a bacterial growth medium*: Specifically, it is a streak plate; the orange lines and dots are formed by bacterial colonies.. A growth medium or culture medium is a solid, liquid, or semi-solid designed to support the growth of a population of microorganisms or cells via the process of cell proliferation [1] or small plants like the moss Physcomitrella patens. [2]
Bacillus mycoides is a bacterium of the genus Bacillus.Like other Bacillus species, B. mycoides is Gram positive, rod-shaped, and forms spores. B. mycoides is distinguished from other Bacillus species by its unusual growth on agar plates, where it forms expansive hairy colonies with characteristic swirls.
Pediococcus acidilactici is a facultative anaerobe that grows well on de Man, Rogosa, Sharpe agar of an optimum pH of 6.2, with an overnight incubation at 37 and 45 °C (99 and 113 °F). It is also viable at higher temperatures up to 65 °C (149 °F).
The streak plate method is a way to physically separate the microbial population, and is done by spreading the inoculate back and forth with an inoculating loop over the solid agar plate. Upon incubation, colonies will arise and single cells will have been isolated from the biomass. Once a microorganism has been isolated in pure culture, it is ...
Blood agar plates (BAPs) contain mammalian blood (usually sheep or horse), typically at a 5–10% concentration. BAPs are enriched, and differential media is used to isolate fastidious organisms and detect hemolytic activity. β-Hemolytic activity will show lysis and complete digestion of red blood cell contents surrounding a colony.
The spread plate method wherein the sample (in a small volume) is spread across the surface of a nutrient agar plate and allowed to dry before incubation for counting. [11] The membrane filter method wherein the sample is filtered through a membrane filter, then the filter placed on the surface of a nutrient agar plate.