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1st-generation ATP tests are derived from hygiene monitoring uses where samples are relatively free of interferences. 2nd-generation tests are specifically designed for water, wastewater and industrial applications where, for the most part, samples contain a variety of components that can interfere with the ATP assay.
The ATPase assay is a membrane assay that indirectly measures the activity of efflux transporters. ATP Binding Cassette or efflux transporters mediate the transport of substrates across cell membranes against a concentration gradient .
ATP is a molecule found only in and around living cells, and as such it gives a direct measure of biological concentration and health. ATP is quantified by measuring the light produced through its reaction with the naturally occurring enzyme firefly luciferase using a luminometer. The amount of light produced is directly proportional to the ...
A viability assay is an assay that is created to determine the ability of organs, cells or tissues to maintain or recover a state of survival. [2] Viability can be distinguished from the all-or-nothing states of life and death by the use of a quantifiable index that ranges between the integers of 0 and 1 or, if more easily understood, the range ...
The first in vitro metabolic measurement, XF technology non-invasively profiles the metabolic activity of cells in minutes, offering scientists a physiologic cell-based assay for the determination of basal oxygen consumption, glycolysis rates, ATP production, and respiratory capacity in a single experiment to assess mitochondrial dysfunction.
Such ATP-based assays include bioluminescent assays in which ATP is the limiting reagent for the luciferase reaction. [6] Cytotoxicity can also be measured by the sulforhodamine B (SRB) assay, WST assay and clonogenic assay.
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