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A Roux culture bottle, or simply Roux bottle, is a type of laboratory glassware used in biology and related sciences to grow microorganisms or tissue cells. [1]: 5 [2]: 1886 It consists of a bottle of transparent glass [3] or plastic [4] with two closely spaced flat, rectangular, parallel faces and a short neck; of such a design that the bottle ...
Line of T175 cell culture flasks containing red cell culture medium. In biology, a subculture is either a new cell culture or a microbiological culture made by transferring some or all cells from a previous culture to fresh growth medium. This action is called subculturing or passaging the cells. Subculturing is used to prolong the lifespan and ...
These cells may be cells isolated from a donor organism (primary cells) or an immortalised cell line. The cells are bathed in a culture medium, which contains essential nutrients and energy sources necessary for the cells' survival. [8] Thus, in its broader sense, "tissue culture" is often used interchangeably with "cell culture".
Cell culture is a fundamental component of tissue culture and tissue engineering, as it establishes the basics of growing and maintaining cells in vitro. The major application of human cell culture is in stem cell industry, where mesenchymal stem cells can be cultured and cryopreserved for future use. Tissue engineering potentially offers ...
The cells are always grown at 37 degrees Celsius with 95% air and 5% carbon dioxide. It is advised to cultivate the cells in flasks that are coated for cell culture adhesion, this will aid in the differentiation and dendrification of the neuroblastoma. In general the cells are quite robust and will grow in most widely used tissue culture media.
Fernbach flask. A Fernbach flask is a type of flask suited for large volume cell culture where the culture requires a large surface area to volume ratio. Typically, they are baffled on the bottom in order to maximize oxygen transfer to the culture medium when shaken. The flask was named after French biologist Auguste Fernbach (1860-1939). [1]
Volumetric flask is used for preparing liquids with volumes of high precision. It is a flask with an approximately pear-shaped body and a long neck with a circumferential fill line. Dewar flask is a double-walled flask having a near-vacuum between the two walls. These come in a variety of shapes and sizes; some are large and tube-like, others ...
A Spinner is a type of bioreactor which features an impeller, stirrer or similar device to agitate the contents (usually a mixture of cells, medium and products like proteins that can be harvested). [1] The vessels are usually made out of glass or stainless steel with port holes to accommodate sensors, Medium input or gas flow.