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Adherent cells, for example many mammalian cell lines, grow attached to a surface such as the bottom of a cell culture flask or petri dish. These cell types have to be detached from the surface before they can be subcultured. For adherent cells, cell density is normally measured in terms of confluency, the percentage of the growth surface ...
Fernbach flask. A Fernbach flask is a type of flask suited for large volume cell culture where the culture requires a large surface area to volume ratio. Typically, they are baffled on the bottom in order to maximize oxygen transfer to the culture medium when shaken. The flask was named after French biologist Auguste Fernbach (1860-1939). [1]
Cell culture is a fundamental component of tissue culture and tissue engineering, as it establishes the basics of growing and maintaining cells in vitro. The major application of human cell culture is in stem cell industry, where mesenchymal stem cells can be cultured and cryopreserved for future use. Tissue engineering potentially offers ...
A Roux culture bottle, or simply Roux bottle, is a type of laboratory glassware used in biology and related sciences to grow microorganisms or tissue cells. [1]: 5 [2]: 1886 It consists of a bottle of transparent glass [3] or plastic [4] with two closely spaced flat, rectangular, parallel faces and a short neck; of such a design that the bottle ...
These cells may be cells isolated from a donor organism (primary cells) or an immortalised cell line. The cells are bathed in a culture medium, which contains essential nutrients and energy sources necessary for the cells' survival. [8] Thus, in its broader sense, "tissue culture" is often used interchangeably with "cell culture".
Culture conditions such as the cell growth medium and surface on which cells are grown vary widely depending on the specific stem cell line. Different biochemical factors can be added to the medium to control the cell phenotype—for example to keep stem cells in a pluripotent state or to differentiate them to a specific cell type.
For genome-scale motif analysis, in 2016, Wong et al. reported a list of 19,491 DNA motif pairs for K562 cell line on the promoter-enhancer interactions. [69] As a result, they proposed that motif pairing multiplicity (number of motifs that are paired with a given motif) is linked to interaction distance and regulatory region type.
Primary cell culture is the ex vivo culture of cells freshly obtained from a multicellular organism, as opposed to the culture of immortalized cell lines.In general, primary cell cultures are considered more representative of in vivo tissues than cell lines, and this is recognized legally in some countries such as the UK (Human Tissue Act 2004). [1]
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