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The Alexa Fluor family of fluorescent dyes is a series of dyes invented by Molecular Probes, now a part of Thermo Fisher Scientific, and sold under the Invitrogen brand name. Alexa Fluor dyes are frequently used as cell and tissue labels in fluorescence microscopy and cell biology . [ 1 ]
Maleimide-mediated methodologies are among the most used in bioconjugation. [5] [6] Due to fast reactions and high selectivity towards cysteine residues in proteins, a large variety of maleimide heterobifunctional reagents are used for the preparation of targeted therapeutics, assemblies for studying proteins in their biological context, protein-based microarrays, or proteins immobilisation. [7]
The intensity,phasor and lifetime image of cells stained with Alexa 488 and Alexa 555. Multi-exponential cases The semicircle represents all possible single ...
3D dual colour super resolution microscopy with Her2 and Her3 in breast cancer cells, standard dyes: Alexa 488, Alexa 568 - LIMON (SPDM +SMI. Vertico spatially modulated illumination (Vertico-SMI) is the fastest [citation needed] light microscope for the 3D analysis of complete cells in the nanometer range. It is based on two technologies ...
For protein labeling, Cy3 and Cy5 dyes sometimes bear a succinimidyl group to react with amines, or a maleimide group to react with a sulfhydryl group of cysteine residues. Cy5 is sensitive to its electronic environment. Changes in the conformation of the protein it is attached to will produce either enhancement or quenching of the emission.
FluoProbes 488, 547H and 647H are found more photostable [7] that is taken to good account in applications using long illuminations periods (i.e. scanning such as in confocal microscopy), [8] or for longer shelf-life of reagents (i.e. manufacturing diagnostics).
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Historically, fluorophores such as fluorescein, rhodamine, Cy3 and Cy5 have been used in a wide variety of applications. These dyes have limitations for use in microscopy and other applications that require exposure to an intense light source such as a laser, because they photobleach quickly (however, bleaching can be reduced at least 10 fold using oxygen scavenging).
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