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The zero-length carbodiimide crosslinker EDC functions by converting carboxyls into amine-reactive isourea intermediates that bind to lysine residues or other available primary amines. SMCC or its water-soluble analog, Sulfo-SMCC, is commonly used to prepare antibody-hapten conjugates for antibody development.
Succinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate (SMCC) is a heterobifunctional amine-to-sulfhydryl crosslinker, which contains two reactive groups at opposite ends: N-hydroxysuccinimide-ester and maleimide, reactive with amines and thiols respectively.
Gold and Silver were designed for the Game Boy Color, allowing them full color support and more detailed sprites. Other additions that were shown included Pokémon breeding, held items, an in-game gadget known as the PokéGear, a real-time internal clock, and backward compatibility with the previous games in the series.
Pokémon Gold and Silver: Nintendo: Game Boy Color: 2000 Pokémon Crystal: 2002 Pokémon Ruby and Sapphire: Nintendo The Pokémon Company: Game Boy Advance: 2003 Pokémon Box: Ruby and Sapphire: GameCube: 2004 Pokémon FireRed and LeafGreen : Game Boy Advance: Pokémon Emerald: 2005 Drill Dozer: Nintendo 2006 Pokémon Diamond and Pearl ...
[8] [fn 1] Diamond and Pearl also introduce several changes to battle mechanics. In previous generations, Pokémon moves were classified as "physical" or "special" based on their type; for example, all Fire-type moves were special and all Ground-type moves were physical. In Diamond and Pearl, however, moves are categorized into three groups. [9]
Pokémon Diamond and Pearl, released in 2006 for the Nintendo DS, introduced 3D graphics for background elements to the series, while foreground elements retained the classic 2D look. In 2006, Japan began the fourth generation of the franchise with the release of Pokémon Diamond and Pearl for Nintendo DS. The games were released in North ...
Pokémon: Diamond and Pearl is the tenth season of the Pokémon anime series, and the first and titular season of Pokémon the Series: Diamond and Pearl, known in Japan as Pocket Monsters Diamond & Pearl (ポケットモンスター ダイヤモンド&パール, Poketto Monsutā Daiyamondo & Pāru).
Pokémon: Diamond and Pearl: Sinnoh League Victors (advertised as Pokémon: DP Sinnoh League Victors), is the thirteenth season of the Pokémon animated series and the fourth and final season of Pokémon the Series: Diamond and Pearl, known in Japan as Pocket Monsters Diamond & Pearl (ポケットモンスター ダイヤモンド&パール, Poketto Monsutā Daiyamondo & Pāru).