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A set of four fluorescently labelled di-base probes compete for ligation to the sequencing primer. Specificity of the di-base probe is achieved by interrogating every 1st and 2nd base in each ligation reaction. Multiple cycles of ligation, detection and cleavage are performed with the number of cycles determining the eventual read length.
Skilled operators are required for consistent, accurate results. [12] When performed in an accredited diagnostic laboratory, the ABI is a fast, accurate, and painless exam, however these issues have rendered ABI unpopular in primary care offices and symptomatic patients are often referred to specialty clinics [13] due to the perceived ...
The BFAST Web Server can be used to align short reads to reference sequences in both nucleotide space as well as ABI SOLiD color space. Utilities include BFAST alignment, conversion between nucleotide and color space, calculating the a priori power of the alignments, as well as a utility to perform Smith Waterman alignment .
For example, the algorithm QualComp [9] performs lossy compression with a rate (number of bits per quality value) specified by the user. Based on rate-distortion theory results, it allocates the number of bits so as to minimize the MSE (mean squared error) between the original (uncompressed) and the reconstructed (after compression) quality values.
These women have ribcage circumferences differing by 2 inches, but when breast tissue is included the measurements are the same at 38 inches. The result is that the latter woman will appear "bustier" than the former due to the apparent difference in bust to hip ratios (narrower shoulders, more prominent breasts) even though they are both ...
PDF/UA is not a separate file-format but simply a way to use the familiar PDF format invented by Adobe Systems and now standardized as ISO 32000. [5]In general PDF/UA requires Tagged PDF (ISO 32000-1, 14.8), but adds a variety of qualitative requirements, especially regarding semantic correctness of the tags employed: [6] [7]
A PDF file is organized using ASCII characters, except for certain elements that may have binary content. The file starts with a header containing a magic number (as a readable string) and the version of the format, for example %PDF-1.7. The format is a subset of a COS ("Carousel" Object Structure) format. [24]
Results are reported either as the absolute values of the tissue in question (in mmHg) or as a ratio of the tissue in question to the control tissue. The normal oxygen tension in the foot is approximately 60 mmHg, and the normal chest/foot ratio is approximately 0.9. [ 2 ]