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For Salkowski test's procedure one needs a sample, that is to be tested for sterols, as well as chloroform and concentrated sulfuric acid that represent Salkowski's reagent. Usually the solution of chloroform and the sample is prepared first and later treated with concentrated sulfuric (VI) acid.
Ehrlich's reagent or Ehrlich reagent is a reagent containing p-dimethylaminobenzaldehyde (DMAB) and thus can act as an indicator to presumptively identify indoles and urobilinogen. Several Ehrlich tests use the reagent in a medical test ; some are drug tests and others contribute to diagnosis of various diseases or adverse drug reactions .
Since this test uses acetic anhydride and sulfuric acid as reagents, caution must be exercised so as not to receive severe burns. Method: Dissolve one or two crystals of cholesterol in dry chloroform in a dry test tube. Add several drops of acetic anhydride and then 2 drops of concentrated H 2 SO 4 and mix carefully.
Marquis reagent is used as a simple spot-test to presumptively identify alkaloids as well as other compounds. It is composed of a mixture of formaldehyde and concentrated sulfuric acid , which is dripped onto the substance being tested.
Sample preparation for mass spectrometry is used for the optimization of a sample for analysis in a mass spectrometer (MS). Each ionization method has certain factors that must be considered for that method to be successful, such as volume, concentration, sample phase, and composition of the analyte solution. Quite possibly the most important ...
para-Dimethylaminobenzaldehyde is an organic compound containing amine and aldehyde moieties which is used in Ehrlich's reagent and Kovac's reagent to test for indoles. The carbonyl group typically reacts with the electron rich 2-position of the indole but may also react at the C-3 or N-1 positions. [2] It may also be used for determination of ...
Salting out (also known as salt-induced precipitation, salt fractionation, anti-solvent crystallization, precipitation crystallization, or drowning out) [1] is a purification technique that utilizes the reduced solubility of certain molecules in a solution of very high ionic strength.
0.117 g of DMACA, 39 mL of ethanol, 5 mL of conc hydrochloric acid and diluted to 50mL with water [1] 1 g DMACA, 1 mL conc. hydrochloric acid and 99 mL water [2] 1 g DMACA in 99 mL conc. hydrochloric acid. [3] It is primarily used as a histological dye used to detect indoles, particularly for production in cells.