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2. DNA extraction - Wikipedia

   en.wikipedia.org/wiki/DNA_extraction

   DNA extraction is the process of isolating DNA from the cells of an organism isolated from a sample, typically a biological sample such as blood, saliva, or tissue. It involves breaking open the cells, removing proteins and other contaminants, and purifying the DNA so that it is free of other cellular components.

3. Spin column-based nucleic acid purification - Wikipedia

   en.wikipedia.org/wiki/Spin_column-based_nucleic...

   The different stages of the method are lyse, bind, wash, and elute. [1] [2] More specifically, this entails the lysis of target cells to release nucleic acids, selective binding of nucleic acid to a silica membrane, washing away particulates and inhibitors that are not bound to the silica membrane, and elution of the nucleic acid, with the end result being purified nucleic acid in an aqueous ...

4. DNA separation by silica adsorption - Wikipedia

   en.wikipedia.org/wiki/DNA_separation_by_silica...

   The highest DNA adsorption efficiencies occur in the presence of buffer solution with a pH at or below the pKa of the surface silanol groups. The mechanism behind DNA adsorption onto silica is not fully understood; one possible explanation involves reduction of the silica surface's negative charge due to the high ionic strength of the buffer.

5. Differential extraction - Wikipedia

   en.wikipedia.org/wiki/Differential_extraction

   Differential extraction (also known as differential lysis) refers to the process by which the DNA from two different types of cells can be extracted without mixing their contents. The most common application of this method is the extraction of DNA from vaginal epithelial cells and sperm cells from sexual assault cases in order to determine the ...

6. Phenol–chloroform extraction - Wikipedia

   en.wikipedia.org/wiki/Phenol–chloroform_extraction

   This is because DNA is more readily neutralized than RNA. There are some disadvantages of this technique in forensic use. It is time-consuming and uses hazardous reagents. Also, because it is a two-step process involving transfer of reagents between tubes, it is at a greater risk of contamination. [3]

7. Boom method - Wikipedia

   en.wikipedia.org/wiki/Boom_method

   Boom method (aka Boom nucleic acid extraction method) is a solid phase extraction method for isolating nucleic acid from a biological sample. This method is characterized by "absorbing the nucleic acids (NA) to the silica beads".

8. Ethanol precipitation - Wikipedia

   en.wikipedia.org/wiki/Ethanol_precipitation

   In DNA extraction, after separating DNA from other cell constituents in water, DNA is precipitated out of solution by neutralizing it with positively charged ions. The addition of ethanol to the solution is necessary to reduce the polarity of the solvent and allow the positively charged ions to interact with the negatively charged phosphate ...

9. Plasmid preparation - Wikipedia

   en.wikipedia.org/wiki/Plasmid_preparation

   A plasmid preparation is a method of DNA extraction and purification for plasmid DNA. It is an important step in many molecular biology experiments and is essential for the successful use of plasmids in research and biotechnology. [1] [2] Many methods have been developed to purify plasmid DNA from bacteria. [1] [3] During the purification ...