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Microorganisms growing on an agar plate. Sterilization (British English: sterilisation) refers to any process that removes, kills, or deactivates all forms of life (particularly microorganisms such as fungi, bacteria, spores, and unicellular eukaryotic organisms) and other biological agents (such as prions or viruses) present in fluid or on a specific surface or object. [1]
Plate culture. An inoculation needle is used in the transfer of microbial organisms from plate culture to needle by first sterilizing the needle to prevent contaminants. The lid of the agar plate culture is then removed to allow the needle access to the microorganisms cultured on the agar plate.
The pour plate technique is the typical technique used to prepare plate count agars. Here, the inoculum is added to the molten agar before pouring the plate. The molten agar is cooled to about 45 degrees Celsius and is poured using a sterile method into a petri dish containing a specific diluted sample.
like HEPA filter, used in various laboratories and clean rooms to produce lamellar air flow Radiation: •Gamma ray source: used in sterilization of heat-labile products like plastic or rubber syringes, catheters and gloves •X-ray source-do- •Infrared light source-do- •Ultraviolet light source-do- Inspissator
[1] This table displays the steps involved in using a GasPak to create a completely anaerobic environment for incubating an inoculated agar plate. After inoculating the agar plate(s) with bacteria under aseptic conditions, the agar plates are placed in an anaerobic jar that contains components, like the catalyst chamber, that will help facilitate the reaction to eliminate free oxygen and ...
Microtitre plates: mostly used for ELISA: Microtome: cuts prepared specimens for analysis under microscope Nichrome wire loop: used to inoculate test samples into culture media for bacterial or fungal cultures, antibiograms, etc.; sterilized by flaming to red hot before use Petri dish/agar plate: to act as a supporting container to hold the ...
An inoculation loop (also called a smear loop, inoculation wand or microstreaker) is a simple tool used mainly by microbiologists to pick up and transfer a small sample of microorganisms called inoculum from a microbial culture, e.g. for streaking on a culture plate. [1] [2] This process is called inoculation.
The plates are incubated for 12 hours up to several days, depending on the test that is performed. Commonly used types of agar plates include: Red blood cells on an agar plate are used to diagnose infection. On the left is a positive Staphylococcus infection, on the right a positive Streptococcus culture.