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  2. Fluorescence microscope - Wikipedia

    en.wikipedia.org/wiki/Fluorescence_microscope

    A fluorescence microscope is an optical microscope that uses fluorescence instead of, or in addition to, scattering, reflection, and attenuation or absorption, to ...

  3. Fluorescence imaging - Wikipedia

    en.wikipedia.org/wiki/Fluorescence_imaging

    Multicolor fluorescence image of living HeLa cells. Fluorescence imaging is a type of non-invasive imaging technique that can help visualize biological processes taking place in a living organism. Images can be produced from a variety of methods including: microscopy, imaging probes, and spectroscopy.

  4. Microscopy - Wikipedia

    en.wikipedia.org/wiki/Microscopy

    Most fluorescence microscopes are operated in the Epi-illumination mode (illumination and detection from one side of the sample) to further decrease the amount of excitation light entering the detector. See also: total internal reflection fluorescence microscope Neuroscience

  5. Fluorescence-lifetime imaging microscopy - Wikipedia

    en.wikipedia.org/wiki/Fluorescence-lifetime...

    Fluorescence-lifetime imaging microscopy or FLIM is an imaging technique based on the differences in the exponential decay rate of the photon emission of a fluorophore from a sample. It can be used as an imaging technique in confocal microscopy , two-photon excitation microscopy , and multiphoton tomography.

  6. Confocal microscopy - Wikipedia

    en.wikipedia.org/wiki/Confocal_microscopy

    Fluorescence and confocal microscopes operating principle. Confocal microscopy, most frequently confocal laser scanning microscopy (CLSM) or laser scanning confocal microscopy (LSCM), is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of using a spatial pinhole to block out-of-focus light in image formation. [1]

  7. Immunofluorescence - Wikipedia

    en.wikipedia.org/wiki/Immunofluorescence

    IF can additionally be used in combination with other, non-antibody methods of fluorescent staining, e.g., the use of DAPI to label DNA. [10] [11] Examination of immunofluorescence specimens can be conducted utilizing various microscope configurations, including the epifluorescence microscope, confocal microscope, and widefield microscope. [12]

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