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The optical microscope, also referred to as a light microscope, is a type of microscope that commonly uses visible light and a system of lenses to generate magnified images of small objects. Optical microscopes are the oldest design of microscope and were possibly invented in their present compound form in the 17th century.
Microscope image processing is a broad term that covers the use of digital image processing techniques to process, analyze and present images obtained from a microscope. Such processing is now commonplace in a number of diverse fields such as medicine , biological research , cancer research , drug testing , metallurgy , etc.
The schematic of a multifocal plane microscope. Multifocal plane microscopy (MUM), also known as multiplane microscopy or multifocus microscopy, is a form of light microscopy that allows the tracking of the 3D dynamics in live cells at high temporal and spatial resolution by simultaneously imaging different focal planes within the specimen.
Stereo microscope. Optical or light microscopy involves passing visible light transmitted through or reflected from the sample through a single lens or multiple lenses to allow a magnified view of the sample. [11] The resulting image can be detected directly by the eye, imaged on a photographic plate, or captured digitally. The single lens with ...
Medical optical imaging is the use of light as an investigational imaging technique for medical applications, pioneered by American Physical Chemist Britton Chance. Examples include optical microscopy , spectroscopy , endoscopy , scanning laser ophthalmoscopy , laser Doppler imaging , optical coherence tomography , and transdermal optical imaging .
The hologram keeps the information on the amplitude and phase of the field. Several holograms may keep information about the same distribution of light, emitted to various directions. The numerical analysis of such holograms allows one to emulate large numerical aperture, which, in turn, enables enhancement of the resolution of optical microscopy.
Pages in category "Optical microscopy techniques" The following 19 pages are in this category, out of 19 total. This list may not reflect recent changes. 0–9.
Photo-activated localization microscopy (PALM or FPALM) [1] [2] and stochastic optical reconstruction microscopy (STORM) [3] are widefield (as opposed to point scanning techniques such as laser scanning confocal microscopy) fluorescence microscopy imaging methods that allow obtaining images with a resolution beyond the diffraction limit.
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