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In biochemistry, denaturation is a process in which proteins or nucleic acids lose folded structure present in their native state due to various factors, including application of some external stress or compound, such as a strong acid or base, a concentrated inorganic salt, an organic solvent (e.g., alcohol or chloroform), agitation and radiation, or heat. [3]
Melting curve analysis is an assessment of the dissociation characteristics of double-stranded DNA during heating. As the temperature is raised, the double strand begins to dissociate leading to a rise in the absorbance intensity, hyperchromicity.
Nucleic acid melting curve showing hyperchromicity as a function of temperature. Hyperchromicity is the increase of absorbance (optical density) of a material.The most famous example is the hyperchromicity of DNA that occurs when the DNA duplex is denatured. [1]
Denaturation Mapping is a form of optical mapping, first described in 1966. It is used to characterize DNA molecules without the need for amplification or sequencing . It is based on the differences between the melting temperatures of AT-rich and GC-rich regions. [ 1 ]
The process of DNA denaturation can be used to analyze some aspects of DNA. Because cytosine / guanine base-pairing is generally stronger than adenine / thymine base-pairing, the amount of cytosine and guanine in a genome is called its GC-content and can be estimated by measuring the temperature at which the genomic DNA melts. [2]
Denaturation (biochemistry), a structural change in macromolecules caused by extreme conditions; Denaturation (fissile materials), transforming fissile materials so that they cannot be used in nuclear weapons; Denaturation (food), intentional adulteration of food or drink rendering it unfit for consumption while remaining suitable for other uses
The word kame is a variant of comb (kame, or kaim is the Old Scottish word meaning 'comb'), which has the meaning "crest" among others. [2] The geological term was introduced by Thomas Jamieson in 1874. [3] According to White, "kames were formed by meltwater which deposited more or less washed material at irregular places in and along melting ice.
[2] neatline A line separating the main body of a map from the map's margin. On a standard quadrangle map, the neatlines are the meridians and parallels delimiting the quadrangle. [3] neck 1. A narrow stretch of land with water on each side, e.g. an isthmus or promontory. [4] 2. A narrow stretch of woodland or of ice. [4] 3.