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An R F value will always be in the range 0 to 1; if the substance moves, it can only move in the direction of the solvent flow, and cannot move faster than the solvent. For example, if particular substance in an unknown mixture travels 2.5 cm and the solvent front travels 5.0 cm, the retardation factor would be 0.50.
The response factor can be expressed on a molar, volume or mass [1] basis. Where the true amount of sample and standard are equal: = where A is the signal (e.g. peak area) and the subscript i indicates the sample and the subscript st indicates the standard. [2]
Another function is the multispot response function (MRF) as developed by De Spiegeleer et al.{Analytical Chemistry (1987):59(1),62-64} It is based also of differences product. This function always lies between 0 and 1. When two RF values are equal, it is equal to 0, when all RF values are equal-spread, it is equal to 1.
The relative mobility (called Rf value or Rm value) is defined as the distance migrated by the protein band divided by the distance migrated by the buffer front. The distances are each measured from the beginning of the separation gel. The migration of the buffer front roughly corresponds to the migration of the dye contained in the sample buffer.
Rf factor may refer to: Chemistry. Retardation factor, a variable measured in chromatography; Biology. ...
The constants listed here are known values of physical constants expressed in SI units; that is, physical quantities that are generally believed to be universal in nature and thus are independent of the unit system in which they are measured. Many of these are redundant, in the sense that they obey a known relationship with other physical ...
Values for other compounds may be found in a variety of available reviews and monographs. [2]: 551ff [21] [page needed] [22]: 1121ff [23] [page needed] [24] Critical discussions of the challenges of measurement of log P and related computation of its estimated values (see below) appear in several reviews. [11] [24]
Molar refractivity, [1] [2], is a measure of the total polarizability of a mole of a substance.. For a perfect dielectric which is made of one type of molecule, the molar refractivity is proportional to the polarizability of a single molecule of the substance.