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Endohydrolysis of (1→3)- or (1→4)-linkages in β-D-glucans when the glucose residue whose reducing group is involved in the linkage to be hydrolysed is itself substituted at C-3 Substrates include laminarin , lichenin and cereal D - glucans .
Cellulose is a polymer composed of β-1,4-linked glucosyl residues. β-glucosidases, cellulases (endoglucanases), cellobiosidases (exoglucanases) are required by a number of organisms to consume it. These enzymes are powerful tools for degradation of plant cell walls by pathogens and other organisms consuming plant biomass. β‑glucosidases ...
It catalyses endohydrolysis of (1→4)-β-D-galactosidic linkages in keratan sulfate. It hydrolyses the 1,4-β- D -galactosyl linkages adjacent to 1,3- N -acetyl-α- D -glucosaminyl residues. References
Mannan endo-1,6-α-mannosidase (EC 3.2.1.101, exo-1,6-β-mannanase, endo-α-1→6-D-mannanase, endo-1,6-β-mannanase, mannan endo-1,6-β-mannosidase, 1,6-α-D-mannan mannanohydrolase) is an enzyme with systematic name 6-α-D-mannan mannanohydrolase. [1] [2] [3] It catalyses the random hydrolysis of (1→6)-α-D-mannosidic linkages in unbranched ...
Glucan endo-1,6-β-glucosidase (EC 3.2.1.75, endo-1,6-β-glucanase, β-(1→6)-β-D-glucanase, β-1,6-glucanase-pustulanase, β-1,6-glucan hydrolase, β-1,6-glucan 6-glucanohydrolase, 1,6-β-D-glucan glucanohydrolase) is an enzyme with systematic name 6-β-D-glucan glucanohydrolase. [1]
The addition of 0.1 M 2-mercaptoethanol highly increases enzyme activity against glycoproteins containing inter- or intra-molecular disulfide bridges, unlike detergents like Triton X-100, n- Octylglucoside, or zwitterionic detergents.
The SR contains ion channel pumps, within its membrane that are responsible for pumping Ca 2+ into the SR. As the calcium ion concentration within the SR is higher than in the rest of the cell, the calcium ions will not freely flow into the SR, and therefore pumps are required, that use energy, which they gain from a molecule called adenosine triphosphate (ATP).
In one study, seven-membered rings were constructed in a tandem 5-exo-dig addition reaction / Claisen rearrangement: [6] A 6-endo-dig pattern was observed in an allene - alkyne 1,2-addition / Nazarov cyclization tandem catalysed by a gold compound: [7] A 5-endo-dig ring closing reaction was part of a synthesis of (+)-Preussin: [8]