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H&E is the combination of two histological stains: hematoxylin and eosin. The hematoxylin stains cell nuclei a purplish blue, and eosin stains the extracellular matrix and cytoplasm pink, with other structures taking on different shades, hues, and combinations of these colors.
Hematoxylin staining shown as "basophilic" at top, seen with dual staining with hematoxylin and eosin (H&E). Haematoxylin stain is commonly followed (or counterstained) with another histologic stain, eosin. [10] [11] [1] When paired, this staining procedure is known as H&E staining, and is one of the most commonly used combinations in histology.
Main staining types when using hematoxylin and eosin (H&E). A Basophil granulocyte stains dark purple upon H&E staining. Basophilic is a technical term used by pathologists. It describes the appearance of cells, tissues and cellular structures as seen through the microscope after a histological section has been stained with a basic dye.
Eosin is usually combined with a stain called hematoxylin to produce a hematoxylin- and eosin-stained section (also called an H&E stain, HE or H+E section). It is the most widely used histological stain for a medical diagnosis. [3] When a pathologist examines a biopsy of a suspected cancer, they will stain the biopsy with H&E.
The trichrome is applied by immersion of the fixated sample into Weigert's iron hematoxylin, and then three different solutions, labeled A, B, and C: Weigert's hematoxylin is a sequence of three solutions: ferric chloride in diluted hydrochloric acid, hematoxylin in 95% ethanol, and potassium ferricyanide solution alkalized by sodium borate.
It stains some tissue in contrasting colors in a way similar to haematoxylin and eosin stain, as phosphotungstic acid binds to tissue proteins. It is used to show gliosis in the central nervous system, tumours of skeletal muscles, and fibrin deposits in lesions.
Hematoxylin bodies occur in systemic lupus erythematosus. The hematoxylin body may be green, blue, or purple with the Papanicolaou stain and magenta with Romanowsky stains . [ 4 ] The material has a positive Feulgen stain reaction, which is typical of DNA . [ 5 ]
Eosin Y is commonly used as the red dye in red inks. It is commonly used in histology, most notably in the H&E (Haematoxylin and Eosin) stain. [1] Eosin Y is also widely used in the Papanicolaou stain (or Pap stain used in the Pap test) and the Romanowsky type cytologic stains.
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