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Mannitol salt agar or MSA is a commonly used selective and differential growth medium in microbiology. It encourages the growth of a group of certain bacteria while inhibiting the growth of others. [ 1 ]
Mannitol motility medium is a bacterial growth medium used to detect the ability of bacteria to ferment mannite and produce nitrogen gas; and to indicate the motility of the organism. [ 1 ] Composition
According to laboratory fermentation tests, P. vulgaris ferments glucose and amygdalin, but does not ferment mannitol or lactose.P. vulgaris also tests positive for the methyl red (mixed acid fermentation) test and is also an extremely motile organism.
The solution remaining yellow (pH = 6.2 or above) indicates a negative test, meaning the butanediol fermentation is used. The VP test uses alpha-naphthol and potassium hydroxide to test for the presence of acetylmethylcarbinol (acetoin), an intermediate of the 2,3-butanediol fermentation pathway. After adding both reagents, the tube is shaken ...
Mannitol is used intravenously to reduce acutely raised intracranial pressure until more definitive treatment can be applied, [16] e.g., after head trauma. While mannitol injection is the mainstay for treating high pressure in the skull after a bad brain injury, it is no better than hypertonic saline as a first-line treatment.
It is designed to selectively isolate gram-negative and enteric (normally found in the intestinal tract) bacteria and differentiate them based on lactose fermentation. [1] Lactose fermenters turn red or pink on MacConkey agar, and nonfermenters do not change color.
Klebsiella aerogenes, [2] previously known as Enterobacter aerogenes, is a Gram-negative, oxidase-negative, catalase-positive, citrate-positive, indole-negative, rod-shaped bacterium. [3]
The plates are incubated for 12 hours up to several days, depending on the test that is performed. Commonly used types of agar plates include: Red blood cells on an agar plate are used to diagnose infection. On the left is a positive Staphylococcus infection, on the right a positive Streptococcus culture.