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The majority of biological samples that are used in research are kept in a buffer solution, often phosphate buffered saline (PBS) at pH 7.4. In industry, buffering agents are used in fermentation processes and in setting the correct conditions for dyes used in colouring fabrics. They are also used in chemical analysis [5] and calibration of pH ...
Buffering HF with NH 4 F results in a solution with a more stable pH; thus, more stable concentrations of HF and HF − 2, and a more stable etch rate. [2] Some oxides produce insoluble products in HF solutions. Thus, HCl may be added to BHF solutions in order to dissolve these insoluble products and produce a higher quality etch. [3]
The following formulas can be used to calculate the volumes of solute (V solute) and solvent (V solvent) to be used: [1] = = where V total is the desired total volume, and F is the desired dilution factor number (the number in the position of F if expressed as "1/F dilution factor" or "xF dilution"). However, some solutions and mixtures take up ...
The useful buffer range for tris (pH 7–9) coincides with the physiological pH typical of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory. Tris is also used as a primary standard to standardize acid solutions for chemical analysis.
The buffering region is dependent upon the pKa, and is typically +/- 1.0 pH units of the pKa. The pKa of KHP is 5.4, so its pH buffering range would be 4.4 to 6.4; however, due to the presence of the second acidic group that bears the potassium ion, the first pKa also contributes to the buffering range well below pH 4.0, which is why KHP is a ...
Disodium pyrophosphate or sodium acid pyrophosphate (SAPP) [1] is an inorganic compound with the chemical formula Na 2 H 2 P 2 O 7. It consists of sodium cations (Na +) and dihydrogen pyrophosphate anions (H 2 P 2 O 2− 7). It is a white, water-soluble solid that serves as a buffering and chelating agent, with many applications in the food ...
TE buffer is a commonly used buffer solution in molecular biology, especially in procedures involving DNA, cDNA or RNA. "TE" is derived from its components: Tris, a common pH buffer, and EDTA, a molecule that chelates cations like Mg 2+. The purpose of TE buffer is to solubilize DNA or RNA, while protecting it from degradation.
TAPS ([tris(hydroxymethyl)methylamino]propanesulfonic acid) is a chemical compound commonly used to make buffer solutions. It can bind divalent cations, including Co(II) and Ni(II). [1] TAPS is effective to make buffer solutions in the pH range 7.7–9.1, since it has a pK a value of 8.44 (ionic strength I = 0, 25 °C). [2]