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  2. Reversed-phase chromatography - Wikipedia

    en.wikipedia.org/wiki/Reversed-phase_chromatography

    Reversed-phase liquid chromatography (RP-LC) is a mode of liquid chromatography in which non-polar stationary phase and polar mobile phases are used for the separation of organic compounds. [ 1 ] [ 2 ] [ 3 ] The vast majority of separations and analyses using high-performance liquid chromatography (HPLC) in recent years are done using the ...

  3. Micellar liquid chromatography - Wikipedia

    en.wikipedia.org/wiki/Micellar_liquid_chromatography

    Reverse phase high-performance liquid chromatography (RP-HPLC) involves a non-polar stationary phase, often a hydrocarbon chain, and a polar mobile or liquid phase. The mobile phase generally consists of an aqueous portion with an organic addition, such as methanol or acetonitrile .

  4. Chromatography - Wikipedia

    en.wikipedia.org/wiki/Chromatography

    Liquid chromatography (LC) is a separation technique in which the mobile phase is a liquid. It can be carried out either in a column or a plane. Present day liquid chromatography that generally utilizes very small packing particles and a relatively high pressure is referred to as high-performance liquid chromatography.

  5. High-performance liquid chromatography - Wikipedia

    en.wikipedia.org/wiki/High-performance_liquid...

    Aqueous normal-phase chromatography (ANP) is also called hydrophilic interaction liquid chromatography (HILIC). [34] This is a chromatographic technique which encompasses the mobile phase region between reversed-phase chromatography (RP) and organic normal phase chromatography (ONP).

  6. Hydrophilic interaction chromatography - Wikipedia

    en.wikipedia.org/wiki/Hydrophilic_interaction...

    Hydrophilic interaction chromatography (or hydrophilic interaction liquid chromatography, HILIC) [1] is a variant of normal phase liquid chromatography that partly overlaps with other chromatographic applications such as ion chromatography and reversed phase liquid chromatography. HILIC uses hydrophilic stationary phases with reversed-phase ...

  7. Monolithic HPLC column - Wikipedia

    en.wikipedia.org/wiki/Monolithic_HPLC_column

    The roots of liquid chromatography extend back over a century ago to 1900, when Russian botanist Mikhail Tsvet began experimenting with plant pigments in chlorophyll. [5] [circular reference] He noted that, when a solvent was applied, distinct bands appeared that migrated at different rates along a stationary phase.

  8. Liquid chromatography–mass spectrometry - Wikipedia

    en.wikipedia.org/wiki/Liquid_chromatography...

    The practice of LC can be divided into five categories, i.e., adsorption chromatography, partition chromatography, ion-exchange chromatography, size-exclusion chromatography, and affinity chromatography. Among these, the most widely used variant is the reverse-phase (RP) mode of the partition chromatography technique, which makes use of a ...

  9. Reversed-phase liquid chromatography - Wikipedia

    en.wikipedia.org/?title=Reversed-phase_liquid...

    Pages for logged out editors learn more. Contributions; Talk; Reversed-phase liquid chromatography

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