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Modern HPLC systems have been improved to work at much higher pressures, and therefore are able to use much smaller particle sizes in the columns (<2 μm). These "ultra high performance liquid chromatography" systems or UHPLCs, which could also be known as ultra high pressure chromatography systems, [53] can work at up to 120 MPa (17,405 lbf/in ...
A monolithic HPLC column, or monolithic column, is a column used in high-performance liquid chromatography (HPLC). The internal structure of the monolithic column is created in such a way that many channels form inside the column. The material inside the column which separates the channels can be porous and functionalized.
Chromatography columns of different types are used in both gas and liquid chromatography: Liquid chromatography: Traditional chromatography columns were made of glass. Modern columns are mostly made of borosilicate glass, acrylic glass or stainless steel. To prevent the stationary phase from leaking out of the column interior a polymer ...
Fast protein liquid chromatography (FPLC) is a form of liquid chromatography that is often used to analyze or purify mixtures of proteins. As in other forms of chromatography, separation is possible because the different components of a mixture have different affinities for two materials, a moving fluid (the mobile phase) and a porous solid (the stationary phase).
Size-exclusion approaches have been described in both high-performance liquid chromatography (HPLC) based and spin column formats. [19] In either case, a mixture of unbound ligands, proteins, ligand-protein complexes is passed through a column of porous beads.
The behavior of fluids and their control in open microchannels came into focus around 2005 [11] and applied in air-to-liquid sample collection [12] [13] and chromatography. [14] In open microfluidics , at least one boundary of the system is removed, exposing the fluid to air or another interface (i.e. liquid).
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