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A fluorescence microscope is an optical microscope that uses fluorescence instead of, or in addition to, scattering, reflection, and attenuation or absorption, to study the properties of organic or inorganic substances.
FCCS is an extension of the fluorescence correlation spectroscopy (FCS) method that uses two fluorescent molecules instead of one that emits different colours. The technique measures coincident green and red intensity fluctuations of distinct molecules that correlate if green and red labelled particles move together through a predefined confocal volume. [2]
A basic diagram of a fluorescence correlation spectroscopy instrument. The typical FCS setup consists of a laser line (wavelengths ranging typically from 405–633 nm (), and from 690–1100 nm (pulsed)), which is reflected into a microscope objective by a dichroic mirror.
In optics, a relay lens is a lens or a group of lenses that receives the image from the objective lens and relays it to the eyepiece. Relay lenses are found in refracting telescopes , endoscopes , and periscopes to optically manipulate the light path , extend the length of the whole optical system , and usually serve the purpose of inverting ...
However, the lobes of a Bessel functions carry as much energy as the central spot, resulting in illumination out of the depth of field of the observation objective. Lattice light sheet microscopy aims at reducing the intensity of the outer lobes of the Bessel functions by destructive interference.
The optical microscope, also referred to as a light microscope, is a type of microscope that commonly uses visible light and a system of lenses to generate magnified images of small objects. Optical microscopes are the oldest design of microscope and were possibly invented in their present compound form in the 17th century.
Two-photon excitation microscopy of mouse intestine.Red: actin.Green: cell nuclei.Blue: mucus of goblet cells.Obtained at 780 nm using a Ti-sapphire laser.. Two-photon excitation microscopy (TPEF or 2PEF) is a fluorescence imaging technique that is particularly well-suited to image scattering living tissue of up to about one millimeter in thickness.
A scanning transmission electron microscope (STEM) is a type of transmission electron microscope (TEM). Pronunciation is [stɛm] or [ɛsti:i:ɛm]. As with a conventional transmission electron microscope (CTEM), images are formed by electrons passing through a sufficiently thin specimen. However, unlike CTEM, in STEM the electron beam is focused ...