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Durham tubes are used in microbiology to detect production of gas by microorganisms. They are simply smaller test tubes inserted upside down in another test tube so they are freely movable. The culture media to be tested is then added to the larger tube and sterilized , which also eliminates the initial air gap produced when the tube is ...
Cragie tube: see link: Desiccator: to dry things Durham's tube: used to detect gas production in sugar fermentation media; the tube is placed in an inverted fashion so that gases produced get trapped in it and do not float away to the surface Gas-pak: releases gases to remove oxygen from a closed container, usually for anaerobiosis: Glass slide
The definition of fermentation has evolved over the years. [1] The most modern definition is catabolism, where organic compounds are both the electron donor and acceptor. [1] [2] A common electron donor is glucose, and pyruvate is a common electron acceptor.
A layer of mineral oil is added to the top of the deep in one of the tubes to create anaerobic conditions. Oil is not added to the other tube to allow for aerobic conditions. The tubes are then incubated for 24–48 hours. If the medium in the anaerobic tube turns yellow, then the bacteria are fermenting glucose.
Stab cultures are similar to agar plates, but are formed by solid agar in a test tube. Bacteria is introduced via an inoculation needle or a pipette tip being stabbed into the center of the agar. Bacteria grow in the punctured area. [11] Stab cultures are most commonly used for short-term storage or shipment of cultures.
Aerobic and anaerobic bacteria can be identified by growing them in test tubes of thioglycollate broth: 1: Obligate aerobes need oxygen because they cannot ferment or respire anaerobically. They gather at the top of the tube where the oxygen concentration is highest.
They gather in the upper part of the test tube but not the very top. 5: Aerotolerant organisms do not require oxygen as they metabolise energy anaerobically. Unlike obligate anaerobes however, they are not poisoned by oxygen. They can be found evenly spread throughout the test tube. Aerotolerant anaerobes use fermentation to produce ATP.
Microbiology (from Ancient Greek μῑκρος (mīkros) 'small' βίος (bíos) 'life' and -λογία () 'study of') is the scientific study of microorganisms, those being of unicellular (single-celled), multicellular (consisting of complex cells), or acellular (lacking cells).