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For bright-field microscopy, negative staining is typically performed using a black ink fluid such as nigrosin and India ink.The specimen, such as a wet bacterial culture spread on a glass slide, is mixed with the negative stain and allowed to dry.
India ink (British English: Indian ink; [1] also Chinese ink) is a simple black or coloured ink once widely used for writing and printing and now more commonly used for drawing and outlining, especially when inking comic books and comic strips. India ink is also used in medical applications.
This can be achieved by smearing the sample onto the slide and then applying nigrosin (a black synthetic dye) or India ink (an aqueous suspension of carbon particles). After drying, the microorganisms may be viewed in bright field microscopy as lighter inclusions well-contrasted against the dark environment surrounding them. [ 6 ]
Structure of a major component of the dye nigrosin. In staining dyes, nigrosin (CI 50415, Solvent black 5) is a mixture of black synthetic dyes made by heating a mixture of nitrobenzene, aniline, and hydrochloric acid in the presence of copper or iron. [1] Related to induline, it is a mixture of phenazine-based compounds.
India ink was invented in China, [11] [12] though materials were often traded from India, hence the name. [ 11 ] [ 12 ] The traditional Chinese method of making the ink was to grind a mixture of hide glue, carbon black , lampblack, and bone black pigment with a pestle and mortar , then pour it into a ceramic dish to dry. [ 11 ]
Image Source: Getty Images. Why 2025 could be a pivotal year for AMD. Much of the reason why Nvidia experienced such enormous growth in its data center business stems from the fact that the ...
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