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The Griess test is an analytical chemistry test which detects the presence of nitrite ion in solution. One of its most important uses is the determination of nitrite in drinking water . The Griess diazotization reaction, on which the Griess reagent relies, was first described in 1858 by Peter Griess .
Colorimetric analysis is a method of determining the concentration of a chemical element or chemical compound in a solution with the aid of a color reagent.It is applicable to both organic compounds and inorganic compounds and may be used with or without an enzymatic stage.
Colorimetric assays use reagents that undergo a measurable color change in the presence of the analyte. They are widely used in biochemistry to test for the presence of enzymes, specific compounds, antibodies, hormones and many more analytes. For example, para-Nitrophenylphosphate is converted into a yellow product by alkaline phosphatase enzyme.
Note that the presence of nitrite ions will interfere with this test. [ 4 ] The overall reaction is the reduction of the nitrate ion to nitric oxide by iron(II), which is oxidised to iron(III), followed by the formation of nitrosyl ferrous sulfate between the nitric oxide and the remaining iron(II), where nitric oxide is reduced to NO − .
A nitrite test is a standard component of a urinary test strip. A positive test for nitrites in the urine is called nitrituria. This test is commonly used in diagnosing urinary tract infections (UTIs). A positive nitrite test indicates that the cause of the UTI is a Gram-negative organism, most commonly Escherichia coli. The reason for nitrites ...
A sample is injected into a flowing carrier solution stream that is forced by a peristaltic pump. The injection of the sample is done under controlled dispersion in known volumes. The carrier solution and sample then meet at mixing points with reagents and react. The reaction time is controlled by a pump and reaction coil.
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