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where L is the column length and N the number of theoretical plates. [5] The relation between plate number and peak width at the base is given by N = 16 ⋅ ( t R W b ) 2 {\displaystyle N=16\cdot \left({\frac {t_{R}}{W_{b}}}\right)^{2}\,} .
Like other forms of chromatography, increasing the column length enhances resolution, and increasing the column diameter increases column capacity. Proper column packing is important for maximum resolution: An over-packed column can collapse the pores in the beads, resulting in a loss of resolution.
In order to calculate the average and standard deviation from aggregate data, it is necessary to have available for each group: the total of values (Σx i = SUM(x)), the number of values (N=COUNT(x)) and the total of squares of the values (Σx i 2 =SUM(x 2)) of each groups.
The variance per unit length of the column is taken as the ratio of the column length to the column efficiency in theoretical plates. The van Deemter equation is a hyperbolic function that predicts that there is an optimum velocity at which there will be the minimum variance per unit column length and, thence, a maximum efficiency. The van ...
Fig. 2: Column effective length factors for Euler's critical load. In practical design, it is recommended to increase the factors as shown above. The following assumptions are made while deriving Euler's formula: [3] The material of the column is homogeneous and isotropic. The compressive load on the column is axial only.
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Lyft stock soared as much as 30% Thursday as the company’s turnaround plan pushed ridership to an all-time high.The ridesharing company posted a record 217 million rides for the quarter ended ...