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  2. Phage display - Wikipedia

    en.wikipedia.org/wiki/Phage_display

    The display of cDNA libraries via phage display is an attractive alternative to the yeast-2-hybrid method for the discovery of interacting proteins and peptides due to its high throughput capability. [ 34 ] pVI has been used preferentially to pVIII and pIII for the expression of cDNA libraries because one can add the protein of interest to the ...

  3. Creative Biolabs - Wikipedia

    en.wikipedia.org/wiki/Creative_Biolabs

    Creative Biolabs, Inc. is a life-science company which produces and supplies biotech products and services for early drug discovery and development, including various phage display libraries [1] such as pre-made libraries, [2] phage display services, [3] [4] antibody sequencing, [5] and antibody humanization. [6]

  4. Methods to investigate protein–protein interactions - Wikipedia

    en.wikipedia.org/wiki/Methods_to_investigate...

    Phage display is used for the high-throughput screening of protein interactions. In-vivo crosslinking of protein complexes using photo-reactive amino acid analogs was introduced in 2005 by researchers from the Max Planck Institute [ 5 ] In this method, cells are grown with photoreactive diazirine analogs to leucine and methionine , which are ...

  5. MorphoSys - Wikipedia

    en.wikipedia.org/wiki/MorphoSys

    MorphoSys’ main technology is HuCAL (Human Combinatorial Antibody Library), which is a collection of more than ten billion fully human antibodies in the form of a phage display bank and a system for their optimization. [27] Another technology recently developed is the OkapY bispecific antibody technology.

  6. mRNA display - Wikipedia

    en.wikipedia.org/wiki/MRNA_display

    mRNA display is a display technique used for in vitro protein, and/or peptide evolution to create molecules that can bind to a desired target. The process results in translated peptides or proteins that are associated with their mRNA progenitor via a puromycin linkage.

  7. Biopanning - Wikipedia

    en.wikipedia.org/wiki/Biopanning

    The final step involves the elution step where the bound phages are eluted through changing of pH or other environment conditions. The end result is the peptides produced by bacteriophage are specific. The resulting filamentous phages can infect gram-negative bacteria once again to produce phage libraries. The cycle can occur many times ...

  8. Two-hybrid screening - Wikipedia

    en.wikipedia.org/wiki/Two-hybrid_screening

    The hybrid expression phagemids can be electroporated into E. coli XL-1 Blue cells which after amplification and infection with VCS-M13 helper phage, will yield a stock of library phage. These phage will each contain one single-stranded member of the phagemid library.

  9. Combinatorial biology - Wikipedia

    en.wikipedia.org/wiki/Combinatorial_biology

    Comparison of natural and synthetic selection processes for peptide generation. In biotechnology, combinatorial biology is the creation of a large number of compounds (usually proteins or peptides) through technologies such as phage display. Similar to combinatorial chemistry, compounds are produced by biosynthesis rather than organic chemistry.

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