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The increase in weight is equal to the amount of liquid displaced by the object, which is the same as the volume of the suspended object times the density of the liquid. [1] The concept of Archimedes' principle is that an object immersed in a fluid is buoyed up by a force equal to the weight of the fluid displaced by the object. [2]
Displacement chromatography is a chromatography technique in which a sample is placed onto the head of the column [n 1] and is then displaced by a solute that is more strongly sorbed than the components of the original mixture.
Air from the tip rises to fill the space left vacant, and the tip air is then replaced by the liquid, which is drawn up into the tip and thus available for transport and dispensing elsewhere. When the pipette knob is pressed on an air displacement pipette, the piston inside the instrument moves down to let air out. Air is displaced by the piston.
Here, ρ represents the density of the fluid; u is a characteristic velocity of the system (for instance, the velocity of a swimming particle); l is a characteristic length scale (e.g., the swimmer size); and μ is the viscosity of the fluid.
When an object is immersed in a liquid, the liquid exerts an upward force, which is known as the buoyant force, that is proportional to the weight of the displaced liquid. The sum force acting on the object, then, is equal to the difference between the weight of the object ('down' force) and the weight of displaced liquid ('up' force).
The culture media to be tested is then added to the larger tube and sterilized, which also eliminates the initial air gap produced when the tube is inserted upside down. The culture media typically contains a single substance to be tested with the organism, such as to determine whether an organism can ferment a particular carbohydrate.
The plunger is depressed to both draw up and dispense the liquid. Normal operation consists of depressing the plunger button to the first stop while the pipette is held in the air. The tip is then submerged in the liquid to be transported and the plunger is released in a slow and even manner. This draws the liquid up into the tip.
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