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DESeq2 is a software package in the field of bioinformatics and computational biology for the statistical programming language R. It is primarily employed for the analysis of high-throughput RNA sequencing (RNA-seq) data to identify differentially expressed genes between different experimental conditions.
For example, suppose that the values x are realizations from different Poisson distributions: i.e. the distributions each have different mean values μ. Then, because for the Poisson distribution the variance is identical to the mean, the variance varies with the mean. However, if the simple variance-stabilizing transformation
Instance normalization (InstanceNorm), or contrast normalization, is a technique first developed for neural style transfer, and is also only used for CNNs. [26] It can be understood as the LayerNorm for CNN applied once per channel, or equivalently, as group normalization where each group consists of a single channel:
In another usage in statistics, normalization refers to the creation of shifted and scaled versions of statistics, where the intention is that these normalized values allow the comparison of corresponding normalized values for different datasets in a way that eliminates the effects of certain gross influences, as in an anomaly time series. Some ...
The plot visualizes the differences between measurements taken in two samples, by transforming the data onto M (log ratio) and A (mean average) scales, then plotting these values. Though originally applied in the context of two channel DNA microarray gene expression data, MA plots are also used to visualise high-throughput sequencing analysis.
Simple back-of-the-envelope test takes the sample maximum and minimum and computes their z-score, or more properly t-statistic (number of sample standard deviations that a sample is above or below the sample mean), and compares it to the 68–95–99.7 rule: if one has a 3σ event (properly, a 3s event) and substantially fewer than 300 samples, or a 4s event and substantially fewer than 15,000 ...
To quantile normalize two or more distributions to each other, without a reference distribution, sort as before, then set to the average (usually, arithmetic mean) of the distributions. So the highest value in all cases becomes the mean of the highest values, the second highest value becomes the mean of the second highest values, and so on.
normalization of dimensional quantities (dividing both the RMS difference and the standard deviation of the "test" field by the standard deviation of the observations) so that the "observed" point is plotted at unit distance from the origin along the x-axis, and statistics for different fields (with different units) can be shown in a single plot;