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The trichrome is applied by immersion of the fixated sample into Weigert's iron hematoxylin, and then three different solutions, labeled A, B, and C: Weigert's hematoxylin is a sequence of three solutions: ferric chloride in diluted hydrochloric acid , hematoxylin in 95% ethanol , and potassium ferricyanide solution alkalized by sodium borate .
Trichrome staining is a histological staining method that uses two or more acid dyes in conjunction with a polyacid. Staining differentiates tissues by tinting them in contrasting colours. It increases the contrast of microscopic features in cells and tissues, which makes them easier to see when viewed through a microscope.
Stain Cell, material, and/or structure(s) stained Condition(s) in which stain is positive Actin-specific enolase: Infantile digital fibromatosis: AE1/AE3: Squamous cell carcinoma: Alcian blue: Lipoid proteinosis Papular mucinosis Scleredema Pretibial myxedema Mucopolysaccharidoses: Alizarin red: Calcinosis cutis: Azure A: Mast cell ...
The Wirtz-Conklin stain is a special technique designed for staining true endospores with the use of malachite green dye as the primary stain and safranin as the counterstain. Once stained, they do not decolourize. The addition of heat during the staining process is a huge contributing factor. [15]
Mallory's trichrome stain also called Mallory's Triple Stain is a stain utilized in histology to aid in revealing different macromolecules that make up the cell. It uses the three stains: aniline blue, acid fuchsin, and orange G. As a result, this staining technique can reveal collagen, ordinary cytoplasm, and red blood cells. It is used in ...
3DISCO (stands for “3D imaging of solvent-cleared organs“) [1] is a histological method that make biological samples more transparent (so called “cleared”) by using series of organic solvents for matching the refractive index (RI) of the tissues with that of the surrounding medium.
It is similar to Masson's trichrome stain, but it uses Biebrich scarlet for the plasma stain. It was initially published by Ralph D. Lillie in 1940. [1] It is applied by submerging the fixated sample into the following three solutions: [2] Weigert's iron hematoxylin working solution, Biebrich scarlet solution, and Fast Green FCF solution.
The term "immunostaining" was originally used to refer to the immunohistochemical staining of tissue sections, as first described by Albert Coons in 1941. [1] However, immunostaining now encompasses a broad range of techniques used in histology, cell biology, and molecular biology that use antibody-based staining methods.