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The Jaffe reaction is a colorimetric method used in clinical chemistry to determine creatinine levels in blood and urine. In 1886, Max Jaffe (1841–1911) wrote about its basic principles in the paper Über den Niederschlag, welchen Pikrinsäure in normalem Harn erzeugt und über eine neue Reaction des Kreatinins in which he described the properties of creatinine and picric acid in an alkaline ...
Clinical chemistry laboratory testing utilizes picric acid for the Jaffe reaction to test for creatinine. It forms a colored complex that can be measured using spectroscopy. [29] Picric acid forms red isopurpurate with hydrogen cyanide (HCN). By photometric measurement of the resulting dye, picric acid can be used to quantify hydrogen cyanide. [30]
Chemical structure of the picrate anion. A picrate is a salt containing the anion (O 2 N) 3 C 6 H 2 O − or an ester derivative of the picrate anion. These salts are often produced by reactions of picric acid (2,4,6-trinitrophenol).
As with other picrates, potassium picrate may be produced by the neutralization of picric acid with the corresponding carbonate. As picric acid is barely soluble in water the reaction must be done in an appropriate solvent like methanol. First dissolving the picric acid in methanol and then adding potassium carbonate will result in potassium ...
Ziehl–Neelsen stain (classic and modified bleach types) [5]; Kinyoun stain; For color blind people (or in backgrounds where detecting red bacteria is difficult), Victoria blue can be substituted for carbol fuchsin and picric acid can be used as the counter stain instead of methylene blue, and the rest of the Kinyoun technique can be used.
TNP-ATP is a fluorescent molecule that is able to determine whether a protein binds to ATP, and the constants associated with that binding.It is primarily used in fluorescence spectroscopy, but is also very useful as an acceptor molecule in FRET, and as a fluorescent probe in fluorescence microscopy and X-ray crystallography.
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The tissue swelling effect of acetic acid is balanced by the tissue shrinking effect of picric acid. [4] Hydrated sections of formaldehyde-fixed tissue are usually pre-treated with Bouin solution to obtain correct results in the trichrome stains for contrasting colours in collagenous and cytoplasmic (muscle) fibres. The trichrome methods were ...