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A UV-Vis spectrophotometer is an analytical instrument that measures the amount of ultraviolet (UV) and visible light that is absorbed by a sample. It is a widely used technique in chemistry, biochemistry, and other fields, to identify and quantify compounds in a variety of samples.
Ultraviolet-visible (UV-vis) spectroscopy involves energy levels that excite electronic transitions. Absorption of UV-vis light excites molecules that are in ground-states to their excited-states. [5] Visible region 400–700 nm spectrophotometry is used extensively in colorimetry science. It is a known fact that it operates best at the range ...
It is commonly used for analysis of compounds that do not absorb UV-VIS radiation significantly, such as sugars, antiviral drugs, antibiotics, fatty acids, lipids, oils, phospholipids, polymers, surfactants, terpenoids and triglycerides. [3] [4]
The vast majority of liquid chromatographic systems are equipped with ultraviolet (UV) absorption detectors. The most common UV-Vis detectors used are variable wavelength detectors (VWD), photo diode array detectors (PDA), and diode array detectors (DAD). [4] Variable wavelength detectors decide in advance which wavelength is needed for the ...
By the 1980s, computers were being incorporated into scientific instruments such as Bausch & Lomb's Spectronic 2000 UV–Vis spectrophotometer, to improve data acquisition and provide instrument control. [29] Specialized spectrophotometers designed for specific tasks now tend to be used rather than general "all-purpose machines" like the DU.
This page was last edited on 26 June 2010, at 19:00 (UTC).; Text is available under the Creative Commons Attribution-ShareAlike 4.0 License; additional terms may ...
Ultraviolet–visible spectroscopy (UV–vis) can distinguish between enantiomers by showing a distinct Cotton effect for each isomer. UV–vis spectroscopy sees only chromophores , so other molecules must be prepared for analysis by chemical addition of a chromophore such as anthracene .
UV-Vis absorption SEC is a recent technique that is continuously evolving. However, many advantages have been observed over other techniques. However, many advantages have been observed over other techniques.