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Using FISH for diagnostic purposes has found its purpose when immediate species identification is needed, specifically for the investigation of blood cultures for which FISH is a cheap and easy technique for preliminary rapid diagnosis. [30] FISH can also be used to compare the genomes of two biological species, to deduce evolutionary ...
PSO is originally attributed to Kennedy, Eberhart and Shi [2] [3] and was first intended for simulating social behaviour, [4] as a stylized representation of the movement of organisms in a bird flock or fish school. The algorithm was simplified and it was observed to be performing optimization.
Quantitative Fluorescent in situ hybridization (Q-FISH) is a cytogenetic technique based on the traditional FISH methodology. In Q-FISH, the technique uses labelled (Cy3 or FITC) synthetic DNA mimics called peptide nucleic acid (PNA) oligonucleotides to quantify target sequences in chromosomal DNA using fluorescent microscopy and analysis software.
Flow-FISH was first published in 1998 by Rufer et al. [11] as a modification of another technique for analyzing telomere length, Q-FISH, that employs peptide nucleic acid probes [12] of a 3'-CCCTAACCCTAACCCTAA-5' sequence labeled with a fluorescin fluorophore to stain telomeric repeats on prepared metaphase spreads of cells that have been treated with colcemid, hypotonic shock, and fixation to ...
Probe design for CISH is very similar to that for FISH with differences only in labelling and detection. FISH probes are generally labelled with a variety of different fluorescent tags and can only be detected under a fluorescence microscope, [4] whereas CISH probes are labelled with biotin or digoxigenin [5] and can be detected using a bright-field microscope after other treatment steps have ...
Tsoukalas, who is now in remission, is far from alone. Americans’ pent-up fury with the nation’s health insurance industry burst into the spotlight last week after the fatal shooting of ...
Fish School Search (FSS), proposed by Bastos Filho and Lima Neto in 2008 is, in its basic version, [1] an unimodal optimization algorithm inspired on the collective behavior of fish schools. The mechanisms of feeding and coordinated movement were used as inspiration to create the search operators.
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