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Generally, Benedict's test detects the presence of aldehyde groups, alpha-hydroxy-ketones, and hemiacetals, including those that occur in certain ketoses. In example, although the ketose fructose is not strictly a reducing sugar, it is an alpha-hydroxy-ketone which results to a positive test because the base component of Benedict converts it ...
Reducing disaccharides like lactose and maltose have only one of their two anomeric carbons involved in the glycosidic bond, while the other is free and can convert to an open-chain form with an aldehyde group. The aldehyde functional group allows the sugar to act as a reducing agent, for example, in the Tollens' test or Benedict's test.
This characteristic of reducing sugars is seen through a positive Benedict's Test. Lactose- N -tetraose has the oligosaccharide nomenclature β- D -galactosyl-(1→3)- N -acetyl-β- D -glucosaminyl-(1→3)-β- D -galactosyl-(1→4)- D -glucose, and consists of lactose with an additional lactose- N -biose disaccharide at the non-reducing end.
Red copper(I) oxide then precipitates out of the reaction mixture, which indicates a positive result i.e. that redox has taken place (this is the same positive result as with Benedict's solution). Fehling's test can be used as a generic test for monosaccharides and other reducing sugars (e.g., maltose).
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Glycosuria leads to excessive water loss into the urine with resultant dehydration, a process called osmotic diuresis. Alimentary glycosuria is a temporary condition, when a high amount of carbohydrate is taken, it is rapidly absorbed in some cases where a part of the stomach is surgically removed, the excessive glucose appears in urine ...
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