Search results
Results from the WOW.Com Content Network
miRNA biogenesis in plants differs from animal biogenesis mainly in the steps of nuclear processing and export. Instead of being cleaved by two different enzymes, once inside and once outside the nucleus, both cleavages of the plant miRNA are performed by a Dicer homolog, called Dicer-like1 (DL1). DL1 is expressed only in the nucleus of plant ...
These two proteins homeostatically control miRNA biogenesis by an auto-feedback loop. [16] A 2nt 3' overhang is generated by Drosha in the nucleus recognized by Dicer in the cytoplasm, which couples the upstream and downstream processing events. Pre-miRNA is then further processed by the RNase Dicer into mature miRNAs in the cell cytoplasm.
The RNA-induced silencing complex, or RISC, is a multiprotein complex, specifically a ribonucleoprotein, which functions in gene silencing via a variety of pathways at the transcriptional and translational levels. [1]
Although DCL1, also called Endoribonuclease Dicer homolog 1, is named for its homology with the metazoan protein Dicer, its role in miRNA biogenesis is somewhat different, due to substantial differences in miRNA maturation processes between plants and animals, [2] as well due to additional downstream plant-specific pathways, where DCL1 paralogs ...
The microprocessor complex consists minimally of two proteins: Drosha, a ribonuclease III enzyme; and DGCR8, a double-stranded RNA binding protein. [4] [5] [6] (DGCR8 is the name used in mammalian genetics, abbreviated from "DiGeorge syndrome critical region 8"; the homologous protein in model organisms such as flies and worms is called Pasha, for Partner of Drosha.)
Dicer, also known as endoribonuclease Dicer or helicase with RNase motif, is an enzyme that in humans is encoded by the DICER1 gene.Being part of the RNase III family, Dicer cleaves double-stranded RNA (dsRNA) and pre-microRNA (pre-miRNA) into short double-stranded RNA fragments called small interfering RNA and microRNA, respectively.
The miR-29 microRNA precursor, or pre-miRNA, is a small RNA molecule in the shape of a stem-loop or hairpin. Each arm of the hairpin can be processed into one member of a closely related family of short non-coding RNAs that are involved in regulating gene expression . [ 1 ]
It has been shown that human ACA45 is a bona fide snoRNA that can be processed into a 21-nucleotides-long mature miRNA by the RNAse III family endoribonuclease dicer. [34] This snoRNA product has previously been identified as mmu-miR-1839 and was shown to be processed independently from the other miRNA-generating endoribonuclease drosha. [35]