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The Hopkins-Cole reaction, also known as the glyoxylic acid reaction, is a chemical test used for detecting the presence of tryptophan in proteins. [1] A protein solution is mixed with Hopkins Cole reagent, which consists of glyoxylic acid. Concentrated sulfuric acid is slowly added to form two layers. A purple ring appears between the two ...
This acetate, bound to the active thiol group of coenzyme A, enters the citric acid cycle (TCA cycle) where it is fully oxidized to carbon dioxide. This pathway thus allows cells to obtain energy from fat. To use acetate from fat for biosynthesis of carbohydrates, the glyoxylate cycle, whose initial reactions are identical to the TCA cycle, is ...
The reaction relies on the interaction between glyoxylic acid and the indole ring of the amino acid tryptophan, a structural feature found in most proteins. When proteins are exposed to concentrated sulfuric acid and glyoxylic acid, the indole group undergoes a reaction that produces a highly colored compound.
Glyoxylic acid is one of several ketone- and aldehyde-containing carboxylic acids that together are abundant in secondary organic aerosols. In the presence of water and sunlight, glyoxylic acid can undergo photochemical oxidation. Several different reaction pathways can ensue, leading to various other carboxylic acid and aldehyde products. [31]
Glyoxylate reductase uses NAD(P)H to reduce an oxoacid (glyoxylate) to its corresponding α-hydroxy acid (glycolate). This class of reactions provides an opportunity for the synthesis of chiral hydroxy acids. Such products are of interest in the synthesis of pharmaceuticals, such as anti-obesity compounds and semisynthetic penicillins. [13]
The enzyme malyl-CoA lyase (EC 4.1.3.24) catalyzes the chemical reaction (3 S )-3-carboxy-3-hydroxypropanoyl-CoA ⇌ {\displaystyle \rightleftharpoons } acetyl-CoA + glyoxylate This enzyme belongs to the family of lyases , specifically the oxo-acid-lyases, which cleave carbon-carbon bonds.
“Trump has made clear that going forward he is going to be even less concerned with abiding by the Constitution and federal law than he was the last time he was in the White House, so the courts ...
d -Glucose + 2 [NAD] + + 2 [ADP] + 2 [P] i 2 × Pyruvate 2 × + 2 [NADH] + 2 H + + 2 [ATP] + 2 H 2 O Glycolysis pathway overview The use of symbols in this equation makes it appear unbalanced with respect to oxygen atoms, hydrogen atoms, and charges. Atom balance is maintained by the two phosphate (P i) groups: Each exists in the form of a hydrogen phosphate anion, dissociating to contribute ...