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Whole genome sequencing (WGS) is the process of determining the entirety, or nearly the entirety, of the DNA sequence of an organism's genome at a single time. [2] This entails sequencing all of an organism's chromosomal DNA as well as DNA contained in the mitochondria and, for plants, in the chloroplast .
The whole genome sequencing technique was first applied to the DNA methylation mapping at single nucleotide resolution to Arabidopsis thaliana in 2008, and shortly after in 2009, the first single-base-resolution DNA methylation map of the entire human genome was created using whole genome bisulfite sequencing.
A finished sequence, covering more than 95% of the genome at approximately 99.99% accuracy; Producing a truly high-quality finished sequence by this definition is very expensive. Thus, most human "whole genome sequencing" results are draft sequences (sometimes above and sometimes below the accuracy defined above). [13]
That same year, Complete Genomics debuted its proprietary whole-genome sequencing service for researchers, charging as little as $5,000/genome for bulk orders. [21] In 2010, Illumina introduced its individual genome sequencing service for consumers, who were required to present a doctor's note. The initial price was $50,000/person.
The process of extensive BAC library creation and tiling path selection, however, make hierarchical shotgun sequencing slow and labor-intensive. Now that the technology is available and the reliability of the data demonstrated, [14] the speed and cost efficiency of whole-genome shotgun sequencing has made it the primary method for genome ...
Whole genome sequencing, a laboratory process that determines the complete DNA sequence of an organism's genome at a single time; World Geodetic System, a reference frame for the earth for use in geodesy and navigation; Wideband Global SATCOM, a United States and Australian military communication satellite system
When you eat ultra-processed foods, you starve your microbiome of the nutrients it needs from whole, fresh food. "This can cause leaky gut syndrome and increase inflammation and oxidative stress ...
Non-repetitive sequence assembled Illumina WGS Zea mays (maize) ssp mays B73: Poaceae: Cereal crop: 2.3 Gb: 39,656 [223] 2009 [224] contig N50 40kbp scaffold N50: 76kbp Sanger, 4-6x coverage per BAC Pennisetum glaucum (pearl millet) Poaceae: Sub-Saharan and Sahelian millet species ~1,79 Gb: 38,579: 2017 [225] WGS and bacterial artificial ...
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