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Microtiter plates with 96, 384 and 1536 wells. A microplate, also known as a microtiter plate, microwell plate or multiwell, [1] is a flat plate with multiple "wells" used as small test tubes. The microplate has become a standard tool in analytical research and clinical diagnostic testing laboratories.
The sample with an unknown amount of antigen is immobilized on a solid support (usually a polystyrene microtiter plate) either non-specifically (via adsorption to the surface) or specifically (via capture by another antibody specific to the same antigen, in a "sandwich" ELISA). After the antigen is immobilized, the detection antibody is added ...
Plate readers, also known as microplate readers or microplate photometers, are instruments which are used to detect biological, chemical or physical events of samples in microtiter plates. They are widely used in research, drug discovery , [ 1 ] bioassay validation, quality control and manufacturing processes in the pharmaceutical and ...
A robot arm handles an assay plate. The key labware or testing vessel of HTS is the microtiter plate, which is a small container, usually disposable and made of plastic, that features a grid of small, open divots called wells. In general, microplates for HTS have either 96, 192, 384, 1536, 3456 or 6144 wells.
Polysorbate 20 is also known as Tween 20, a commercial brand name. It is a common detergent used in many buffers for washing nitrocellulose membrane in western blotting and microtiter plate wells in ELISA assays. Tris is a buffer that maintains a pH of 7–9.2.
Performing the suPARnostic ELISA requires two antibodies with high specificity for suPAR. The blood plasma sample from the patient that contains an unknown amount of suPAR is immobilized on the microwells on the clear microtiter plate and a detection antibody forms a complex with suPAR. Between each step the plate is rinsed with a wash buffer ...
Microtiter plates with 96, 384 and 1536 wells. Indirect immunoperoxidase assay (IPA) is a laboratory technique used to detect and titrate viruses that do not cause measurable cytopathic effects and cannot be measured by classical plaque assays. These viruses include human coronavirus 229E and OC43. [1]
A microtiter plate after an MTT assay. Increasing amounts of cells resulted in increased purple colouring. The MTT assay is a colorimetric assay for assessing cell metabolic activity. [1] [2] NAD(P)H-dependent cellular oxidoreductase enzymes may, under defined conditions, reflect the number of viable cells present.
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