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Bromothymol blue (also known as bromothymol sulfone phthalein and BTB) is a pH indicator. It is mostly used in applications that require measuring substances that would have a relatively neutral pH (near 7). A common use is for measuring the presence of carbonic acid in a liquid. It is typically sold in solid form as the sodium salt of the acid ...
Bromothymol blue is the indicator used in the agar, it changes to yellow in case of acid production during fermentation of lactose or changes to deep blue in case of alkalinization. Lactose-positive bacteria build yellow colonies. Bacteria which decarboxylate L-cystine cause an alkaline reaction and build deep blue colonies. [1]
The increase in pH then causes color change in the bromothymol blue indicator, turning it blue. Under neutral conditions the medium remains a green color. The color change to blue is useful because growth on Simmons' citrate agar is often limited and would be hard to observe if it were not for the color change.
When you eat food, it eventually turns that color by the time it exits the body in the form of stool, according to Baltimore colon and rectal surgeon Jeffery Nelson, MD, the surgical director at ...
yellow 0.0 2.0 blue-violet Malachite green (first transition) yellow 0.0 2.0 green Malachite green (second transition) green 11.6 14.0 colorless Thymol blue (first transition) red 1.2 2.8 yellow Thymol blue (second transition) yellow 8.0 9.6 blue Methyl yellow: red 2.9 4.0 yellow Methylene blue: colorless 5.0 9.0 dark blue Bromophenol blue ...
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Yellow coloured (sucrose-fermenting) colonies of Vibrio cholerae on TCBS agar. Approximate amounts per liter [3] Yeast extract 5.0 g; Proteose Peptone 10.0 g; Sodium thiosulfate 10.0 g; Sodium citrate 10.0 g; Ox gall 5.0 g; Sodium cholate 3.0 g; Saccharose 20.0 g; Sodium chloride 10.0 g; Ferric citrate 1.0 g; Bromothymol blue 0.04 g; Thymol ...
It transitions from red to yellow at pH 1.2–2.8 and from yellow to blue at pH 8.0–9.6. It is usually a component of Universal indicator. At wavelength (378 - 382) nm, extinction coefficient > 8000 and at wavelength (298 - 302) nm, the extinction coefficient > 12000. [3]