Search results
Results from the WOW.Com Content Network
In hemocytometry, Türk's solution (or Türk's fluid) is a hematological stain (either crystal violet or aqueous methylene blue) prepared in 99% acetic acid (glacial) [1] and distilled water. The solution destroys the red blood cells and platelets within a blood sample (acetic acid being the main lyzing agent ), and stains the nuclei of the ...
Cell counting is any of various methods for the counting or similar quantification of cells in the life sciences, including medical diagnosis and treatment. It is an important subset of cytometry , with applications in research and clinical practice.
Determining the viable cell count is important for calculating dilutions required for the passaging of cells, as well as determining the size and number of flasks needed during growth time. It is also vital when seeding plates for assays, such as the plaque assay , [ 2 ] because the plates need a known number of live replicating cells for the ...
The LaTeX source code is attached to the PDF file (see imprint). Licensing Permission is granted to copy, distribute and/or modify this document under the terms of the GNU Free Documentation License , Version 1.2 or any later version published by the Free Software Foundation ; with no Invariant Sections, no Front-Cover Texts, and no Back-Cover ...
MTT, a yellow tetrazole, is reduced to purple formazan in living cells. [8] A solubilization solution (usually either dimethyl sulfoxide, an acidified ethanol solution, or a solution of the detergent sodium dodecyl sulfate in diluted hydrochloric acid) is added to dissolve the insoluble purple formazan product into a colored solution.
Lugol's iodine solution is always added after addition of crystal violet to form a stable complex with crystal violet that strengthen the bonds of the stain with the cell wall. [4] Gram staining is almost always the first step in the identification of a bacterial group. While Gram staining is a valuable diagnostic tool in both clinical and ...
Acid fuchsin or fuchsine acid, (also called Acid Violet 19 [1] and C.I. 42685 [1]) is an acidic magenta dye with the chemical formula C 20 H 17 N 3 Na 2 O 9 S 3. It is a sodium sulfonate derivative of fuchsine. Acid fuchsin has wide use in histology, [1] and is one of the dyes used in Masson's trichrome stain. [2]
Used as a mordant in Gram's staining, iodine enhances the entrance of the dye through the pores present in the cell wall/membrane. Lugol's solution or Lugol's iodine (IKI) is a brown solution that turns black in the presence of starches and can be used as a cell stain, making the cell nuclei more visible.