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However, sucrose indirectly produces a positive result with Benedict's reagent if heated with dilute hydrochloric acid prior to the test, although it is modified during this treatment as the acidic conditions and heat break the glycosidic bond in sucrose through hydrolysis. The products of sucrose decomposition are glucose and fructose, both of ...
An example of a positive Seliwanoff’s test. Seliwanoff’s test is a chemical test which distinguishes between aldose and ketose sugars. If the sugar contains a ketone group, it is a ketose. If a sugar contains an aldehyde group, it is an aldose. This test relies on the principle that, when heated, ketoses are more rapidly dehydrated than ...
The sucrose lysis test is a diagnostic laboratory test used for diagnosing paroxysmal nocturnal hemoglobinuria (PNH), as well as for hypoplastic anemias and any hemolytic anemia with an unclear cause. [1] The test works by using sucrose, which creates a low ionic strength environment that allows complement to bind to red blood cells. [1]
Cystine tryptic agar (CTA), also known as cystine trypticase agar, [1] [2] is a growth medium used for the identification of microorganisms. [3]It can be used to determine if organisms can ferment various carbohydrates, including maltose, lactose, and sucrose.
The TSI slant is a test tube that contains agar, a pH-sensitive dye , 1% lactose, 1% sucrose, 0.1% glucose, [2] and sodium thiosulfate and ferrous sulfate or ferrous ammonium sulfate. All of these ingredients are mixed together, heated to sterility, and allowed to solidify in the test tube at a slanted angle.
Some sugars, such as sucrose, do not react with any of the reducing-sugar test solutions. However, a non-reducing sugar can be hydrolyzed using dilute hydrochloric acid . After hydrolysis and neutralization of the acid, the product may be a reducing sugar that gives normal reactions with the test solutions.
Sucrose intolerance can also be caused by irritable bowel syndrome, aging, or small intestine disease (secondary sucrose intolerance). There are specific tests used to help determine if a person has sucrose intolerance. The most accurate test is the enzyme activity determination, which is done by biopsying the small intestine.
Sucrose is a non-reducing sugar, so will not test positive with Benedict's solution. To test for sucrose, the sample is treated with sucrase. The sucrose is hydrolysed into glucose and fructose, with glucose being a reducing sugar, which in turn tests positive with Benedict's solution. [citation needed].
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