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3T3 cells are several cell lines of mouse embryonic fibroblasts. The original 3T3 cell line (3T3-Swiss albino) was established in 1962 by two scientists then at the Department of Pathology in the New York University School of Medicine, George Todaro and Howard Green. Todaro and Green originally obtained their 3T3 cells from Swiss albino mouse ...
The 3T3-L1 cell line is a sub clone that was initially developed from a mouse embryo, from a clonal expansion of Swiss 3T3 cells. [2] [3] In 1962, the original 3T3 cell line that it was established by George Todaro and Howard Green of the New York University School of Medicine. [6]
A photo of human embryonic stem cells (the cell colonies in the center). Spindle cells surrounding the stem cell colony are MEFs. Mouse Embryonic Fibroblasts (MEFs) are a type of fibroblast prepared from mouse embryo. MEFs show a spindle shape when cultured in vitro, a typical feature of fibroblasts. The MEF is a limited cell line.
The 3T3 Neutral Red Uptake (NRU) Phototoxicity Test, approved by the OECD, detects the viability of 3T3 cells after exposure to a chemical in the presence or absence of light. [17] The 3T3 cell line was developed in 1962 and is derived from mouse embryonic fibroblast cells. [18]
Adipogenesis is the formation of adipocytes (fat cells) from stem cells. [1] It involves 2 phases, determination, and terminal differentiation. Determination is mesenchymal stem cells committing to the adipocyte precursor cells, also known as lipoblasts or preadipocytes which lose the potential to differentiate to other types of cells such as ...
Fibroblasts can also migrate slowly over substratum as individual cells, again in contrast to epithelial cells. While epithelial cells form the lining of body structures, fibroblasts and related connective tissues sculpt the "bulk" of an organism. The life span of a fibroblast, as measured in chick embryos, is 57 ± 3 days. [4]
3T3 cells, a cell line established in 1962 by two scientists at the Department of Pathology in the New York University School of Medicine Topics referred to by the same term This disambiguation page lists articles associated with the same title formed as a letter–number combination.
Co-culturing 3T3-L1 preadipocytes in a 3D space with murine endothelial bEND.3 cells can create a vascular-like network assembly with concomitant lipogenesis in perivascular cells (refer to the attached figure). [6] In addition to cell lines, organogenesis of white adipose tissue (WAT) can be simulated from primary cells. [6]
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