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The Griess test is an analytical chemistry test which detects the presence of nitrite ion in solution. One of its most important uses is the determination of nitrite in drinking water. The Griess diazotization reaction, on which the Griess reagent relies, was first described in 1858 by Peter Griess.
Colorimetric analysis is a method of determining the concentration of a chemical element or chemical compound in a solution with the aid of a color reagent.It is applicable to both organic compounds and inorganic compounds and may be used with or without an enzymatic stage.
A nitrite test is a standard component of a urinary test strip. A positive test for nitrites in the urine is called nitrituria. This test is commonly used in diagnosing urinary tract infections (UTIs). A positive nitrite test indicates that the cause of the UTI is a Gram-negative organism, most commonly Escherichia coli. The reason for nitrites ...
Colorimetric assays use reagents that undergo a measurable color change in the presence of the analyte. They are widely used in biochemistry to test for the presence of enzymes, specific compounds, antibodies, hormones and many more analytes. For example, para-Nitrophenylphosphate is converted into a yellow product by alkaline phosphatase enzyme.
Several conventional colorimetric analyses have been miniaturized to function quantitatively in a plate reader, with performance suitable for research purposes. Examples of analyses converted to plate reader methods include several for ammonium , nitrate , nitrite , [ 4 ] urea , [ 5 ] iron(II), [ 6 ] and orthophosphate . [ 7 ]
After a refluxing digestion step, the initial concentration of organic substances in the sample is calculated from a titrimetric or spectrophotometric determination of the oxidant remaining in the sample. As with all colorimetric methods, blanks are used to control for contamination by outside material.
We asked experts what you should say and what you should not say to a friend that has experienced a miscarriage.
Phenol in the Berthelot reagent can be replaced by a variety of phenolic reagents, the most common being sodium salicylate, which is significantly less toxic. [1] This has been used for blood urea nitrogen (BUN) determinations and commonly is used to determine water and soil total and ammonia-N. Replacement of phenol by 2-phenylphenol reduces interferences by a variety of soil and water ...
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