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Cryogenic electron microscopy (cryo-EM) is a cryomicroscopy technique applied on samples cooled to cryogenic temperatures. For biological specimens, the structure is preserved by embedding in an environment of vitreous ice .
CryoTEM image of GroEL suspended in amorphous ice at 50 000 × magnification Structure of Alcohol oxidase from Pichia pastoris by CryoTEM. Transmission electron cryomicroscopy (CryoTEM), commonly known as cryo-EM, is a form of cryogenic electron microscopy, more specifically a type of transmission electron microscopy (TEM) where the sample is studied at cryogenic temperatures (generally liquid ...
Cryogenic electron tomography (cryoET) is an imaging technique used to reconstruct high-resolution (~1–4 nm) three-dimensional volumes of samples, often (but not limited to) biological macromolecules and cells.
Cryo-electron Microscopy (cryo-EM) is the initial technique that allowed scientists to image particles and structures on the surface of viruses. An electron microscope is utilized so electrons illuminate the viruses or bacteria observed. The light is manipulated at different angles and this creates multiple images of the bacterial structure.
Scanning electron cryomicroscopy (CryoSEM) is a form of electron microscopy where a hydrated but cryogenically fixed sample is imaged on a scanning electron microscope's cold stage in a cryogenic chamber. The cooling is usually achieved with liquid nitrogen. [1]
Microcrystal electron diffraction, or MicroED, [1] [2] is a CryoEM method that was developed by the Gonen laboratory in late 2013 at the Janelia Research Campus of the Howard Hughes Medical Institute.
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Recently, cryo-electron microscopy (cryo-EM) became an integral part of the Ernst-Ruska Centre with state-of-the-art cryo-microscopes: 300 kV Titan Krios G4 (operational in Summer 2021) and 200 kV Talos Arctica including Gatan Bioquantum K3 detectors.