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Class I, the intracellular peroxidases, includes: cytochrome c peroxidase (CCP), a soluble protein found in the mitochondrial electron transport chain, where it probably protects against toxic peroxides; ascorbate peroxidase (AP), the main enzyme responsible for hydrogen peroxide removal in chloroplasts and cytosol of higher plants; [4] and ...
As long as the enzyme survives, the reaction of heme with peroxide is catalytic, making this test very sensitive to small quantities of blood present on the test swab. The hemoglobin-catalyzed reduction of peroxide that occurs is shown in the reaction below. The two electrons are supplied by phenolphthalein: HOOH + 2 e − + 2 H + → 2 H 2 O
In whole blood (g/cm 3) In plasma or serum (g/cm 3) Water: ... avg. (range) 5.2 ... Hydrogen ion(pH 7.4) 4 ...
Hydrogen peroxide is a chemical compound with the formula H 2 O 2.In its pure form, it is a very pale blue [5] liquid that is slightly more viscous than water.It is used as an oxidizer, bleaching agent, and antiseptic, usually as a dilute solution (3%–6% by weight) in water for consumer use and in higher concentrations for industrial use.
Heme peroxidases catalyse the H 2 O 2-dependent oxidation of a wide range of different, usually organic, substrates in biology. This enzyme belongs to the family of oxidoreductases, specifically those acting on a peroxide as acceptor (peroxidases). The systematic name of this enzyme class is L-ascorbate:hydrogen-peroxide oxidoreductase.
Spectrophotometry is a tool that hinges on the quantitative analysis of molecules depending on how much light is absorbed by colored compounds. Important features of spectrophotometers are spectral bandwidth (the range of colors it can transmit through the test sample), the percentage of sample transmission, the logarithmic range of sample ...
Reference ranges (reference intervals) for blood tests are sets of values used by a health professional to interpret a set of medical test results from blood samples. Reference ranges for blood tests are studied within the field of clinical chemistry (also known as "clinical biochemistry", "chemical pathology" or "pure blood chemistry"), the ...
The near-infrared (NIR) window (also known as optical window or therapeutic window) defines the range of wavelengths from 650 to 1350 nanometre (nm) where light has its maximum depth of penetration in tissue. [1] Within the NIR window, scattering is the most dominant light-tissue interaction, and therefore the propagating light becomes diffused ...