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The "elution time" of a solute is the time between the start of the separation (the time at which the solute enters the column) and the time at which the solute elutes. In the same way, the elution volume is the volume of eluent required to cause elution. Under standard conditions for a known mix of solutes in a certain technique, the elution ...
Chromatography employs continuous adsorption and desorption on a packed bed of a solid to purify multiple components of a single feed stream. In a laboratory setting, mixture of dissolved materials are typically fed using a solvent into a column packed with an appropriate adsorbent, and due to different affinities for solvent (moving phase ...
Elution of the adsorbed proteins was commonly performed with the eluent flow in the reverse direction; that is, as a conventional packed bed, in order to recover the adsorbed solutes in a smaller volume of eluent. However, a new generation of EBA columns has been developed, which maintain the bed in the expanded state during this phase ...
In any form of chromatography, the rate at which the solute moves down the column is a direct reflection of the percentage of time the solute spends in the mobile phase. To achieve separation in either elution or displacement chromatography, there must be appreciable differences in the affinity of the respective solutes for the stationary phase.
Anion-exchange chromatography is a process that separates substances based on their charges using an ion-exchange resin containing positively charged groups, such as diethyl-aminoethyl groups (DEAE). [2] In solution, the resin is coated with positively charged counter-ions . Anion exchange resins will bind to negatively charged molecules ...
However, the theoretical plate in packed beds, chromatography and other applications is defined as having a height. The empirical formula known as Van Winkle's Correlation can be used to predict the Murphree plate efficiency for distillation columns separating binary systems.
The different stages of the method are lyse, bind, wash, and elute. [1] [2] More specifically, this entails the lysis of target cells to release nucleic acids, selective binding of nucleic acid to a silica membrane, washing away particulates and inhibitors that are not bound to the silica membrane, and elution of the nucleic acid, with the end result being purified nucleic acid in an aqueous ...
In liquid chromatography, the mobile phase velocity is taken as the exit velocity, that is, the ratio of the flow rate in ml/second to the cross-sectional area of the ‘column-exit flow path.’ For a packed column, the cross-sectional area of the column exit flow path is usually taken as 0.6 times the cross-sectional area of the column.