Search results
Results from the WOW.Com Content Network
The Bradford protein assay (also known as the Coomassie protein assay) was developed by Marion M. Bradford in 1976. [1] It is a quick and accurate [2] spectroscopic analytical procedure used to measure the concentration of protein in a solution. The reaction is dependent on the amino acid composition of the measured proteins.
A calibration curve plot showing limit of detection (LOD), limit of quantification (LOQ), dynamic range, and limit of linearity (LOL).. In analytical chemistry, a calibration curve, also known as a standard curve, is a general method for determining the concentration of a substance in an unknown sample by comparing the unknown to a set of standard samples of known concentration. [1]
Bradford was born October 28, 1946, in Rome, Georgia, US, and received his B.A. from Shorter College there in 1967. [1] In 1971 he married Janet Holliday. [1] [8] He obtained his Ph.D. in biochemistry from the University of Georgia in 1975, and his use of the Coomassie Brilliant Blue G-250 dye to detect proteins, which became known as the Bradford assay, was patented in 1976.
[3]: 21–119 Following this testing the sample at 420 nm for specific interaction with ONPG and at 595 for a Bradford Assay the amount of purification can be assessed quantitatively. [ 3 ] : 21–119 In addition to this spectrophotometry can be used in tandem with other techniques such as SDS-Page electrophoresis in order to purify and isolate ...
[1] [2] The list of papers whose results were later retracted or discredited, thus leading to invalid science, is growing. [3] Some errors are introduced when the experimenter's desire for a certain result unconsciously influences selection of data (a problem which is possible to avoid in some cases with double-blind protocols). [4]
An assay (analysis) is never an isolated process, as it must be accompanied with pre- and post-analytic procedures. Both the communication order (the request to perform an assay plus related information) and the handling of the specimen itself (the collecting, documenting, transporting, and processing done before beginning the assay) are pre-analytic steps.
The following is a list of notable unsolved problems grouped into broad areas of physics. [1]Some of the major unsolved problems in physics are theoretical, meaning that existing theories seem incapable of explaining a certain observed phenomenon or experimental result.
BCA protein assay in a 96 well plate. The bicinchoninic acid assay (BCA assay), also known as the Smith assay, after its inventor, Paul K. Smith at the Pierce Chemical Company, [1] now part of Thermo Fisher Scientific, is a biochemical assay for determining the total concentration of protein in a solution (0.5 μg/mL to 1.5 mg/mL), similar to Lowry protein assay, Bradford protein assay or ...