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Example chromatogram showing signal as a function of retention time. In chromatography, resolution is a measure of the separation of two peaks of different retention time t in a chromatogram. [1] [2] [3] [4]
In liquid chromatography, the mobile phase velocity is taken as the exit velocity, that is, the ratio of the flow rate in ml/second to the cross-sectional area of the ‘column-exit flow path.’ For a packed column, the cross-sectional area of the column exit flow path is usually taken as 0.6 times the cross-sectional area of the column.
The partition coefficient principle has been applied in paper chromatography, thin layer chromatography, gas phase and liquid–liquid separation applications. The 1952 Nobel Prize in chemistry was earned by Archer John Porter Martin and Richard Laurence Millington Synge for their development of the technique, which was used for their ...
The thermal conductivity detector (TCD), also known as a katharometer, is a bulk property detector and a chemical specific detector commonly used in gas chromatography. [1] This detector senses changes in the thermal conductivity of the column eluent and compares it to a reference flow of carrier gas. Since most compounds have a thermal ...
The valley definition defines ΔM as the closest spacing of two peaks of equal intensity with the valley (lowest value of signal) between them less than a specified fraction of the peak height. Typical values are 10% or 50%. The value obtained from a 5% peak width is roughly equivalent to a 10% valley. [1]
(For instance, if the first column offers a peak capacity (k 1)of 100 for a 10-minute separation, and the second column offers a peak capacity of 5 (k 2) in a 5-second separation, then the combined peak capacity may approach k 1 × k 2 =500, with the total separation time still ~ 10 minutes). 2D separations have been applied to the analysis of ...
An example base peak chromatogram from an LC-MS analysis. The base peak chromatogram is similar to the TICC, however it monitors only the most intense peak in each spectrum. [4] [3] This means that the base peak chromatogram represents the intensity of the most intense peak at every point in the analysis. Base peak chromatograms often have a ...
The second separation is rapid, allowing the introduction of subsequent fractions from the first column without mutual interference. Dallüge et al. [28] reviewed the principles, advantages and main characteristics of this technique. One of the main advantages is the very high separation power, making the technique ideal for unravelling the ...