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Base line no base line. Variable pathlength absorption spectroscopy uses a determined slope to calculate concentration. As stated above this is a product of the molar absorptivity and the concentration. Since the actual absorbance value is taken at many data points at equal intervals, background subtraction is generally unnecessary.
Cell culture is a fundamental component of tissue culture and tissue engineering, as it establishes the basics of growing and maintaining cells in vitro. The major application of human cell culture is in stem cell industry, where mesenchymal stem cells can be cultured and cryopreserved for future use. Tissue engineering potentially offers ...
The slope of this line is the specific growth rate of the organism, which is a measure of the number of divisions per cell per unit time. [5] The actual rate of this growth (i.e. the slope of the line in the figure) depends upon the growth conditions, which affect the frequency of cell division events and the probability of both daughter cells ...
The slope or pitch of the stairs is the ratio between the rise and the going (not the tread depth, due to the nosing). It is sometimes called the rake of the stairs. The pitch line is the imaginary line along the tip of the nosing of the treads. In the UK, stair pitch is the angle the pitch line makes with the horizontal, measured in degrees.
Primary cell culture is the ex vivo culture of cells freshly obtained from a multicellular organism, as opposed to the culture of immortalized cell lines.In general, primary cell cultures are considered more representative of in vivo tissues than cell lines, and this is recognized legally in some countries such as the UK (Human Tissue Act 2004). [1]
Line of T175 cell culture flasks containing red cell culture medium. In biology, a subculture is either a new cell culture or a microbiological culture made by transferring some or all cells from a previous culture to fresh growth medium. This action is called subculturing or passaging the cells. Subculturing is used to prolong the lifespan and ...
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These cells may be cells isolated from a donor organism (primary cells) or an immortalised cell line. The cells are bathed in a culture medium, which contains essential nutrients and energy sources necessary for the cells' survival. [8] Thus, in its broader sense, "tissue culture" is often used interchangeably with "cell culture".