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Hemolysis is the breakdown of red blood cells. The ability of bacterial colonies to induce hemolysis when grown on blood agar is used to classify certain microorganisms. This is particularly useful in classifying streptococcal species. A substance that causes hemolysis is called a hemolysin.
DTM plate on the right showing color change after two days' growth; Microsporum canis Dermatophyte test medium (DTM) is a specialized agar used in medical mycology. [1] [2] It is based on Sabouraud's dextrose agar with added cycloheximide to inhibit saprotrophic growth, antibiotic to inhibit bacterial growth, and phenol red a pH indicator.
The presence of bubbles in the medium indicates the presence of gaseous nitrogen, produced by the organism. The pattern of color change (localized along the inoculation site vs. dispersed throughout the medium) indicates the motility of the organism. [1]
From left to right solutions of 0.1 M HCl, 3 buffer solutions of pH 3.78, 3 of pH 4.00, 3 of pH 4.62 and NaOH 0.1 M after adding different amounts of bromocresol green (more in darker solutions) In aqueous solution , bromocresol green will ionize to give the monoanionic form (yellow), that further deprotonates at higher pH to give the dianionic ...
Chocolate agar is a type of blood agar plate in which the blood cells have been lysed by heating the cells to 80 °C. It is used for growing fastidious respiratory bacteria, such as Haemophilus influenzae. Chocolate agar is named for its color, and no chocolate is contained in the plate.
However, once the agar has solidified, the pH does not appear to change with temperature but remains at 6.9. For preparation of BCYE + antibiotics, add membrane-filtered antibiotics and mix. For BCYE + albumin agar, dissolve the albumin in distilled water and filter sterilize before addition to the medium. Dispense 20 mL per 15 X 100-mm Petri dish.
To prepare 1 liter of TSB, the following ingredients are dissolved under gentle heat. Adjustments to pH should be made using 1N HCl or 1N NaOH to reach a final target pH of 7.3 ± 0.2 at 25°C. The solution is then autoclaved for 15 minutes at 121°C. [3] Tryptic Soy Agar contains per liter: 17 g pancreatic digest of casein; 3 g peptic digest ...
Tissue culture flasks. RPMI 1640, simply known as RPMI medium, is a cell culture medium commonly used to culture mammalian cells. [1] RPMI 1640 was developed by George E. Moore, Robert E. Gerner, and H. Addison Franklin in 1966 at Roswell Park Comprehensive Cancer Center (formerly known as Roswell Park Memorial Institute), from where it derives its name. [2]